MolNFT v1 · #172272 · protein + RNA

Cryo-EM structure of a late human pre-40S ribosomal subunit - state G

6ZXF · Pre-18S ribosomal RNA: “Cryo-EM structure of a late human pre-40S ribosomal subunit - state G”. Electron microscopy, 3 ligands (ATP, ZN, MG), 20 pockets (best druggability 0.89). MolNFT v1 #172272 on GenesisL1: 3D structure, binding…

PDB: 6ZXF
Type: protein + RNA
Length: 7,414 residues
Chains: 37
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Pre-18S ribosomal RNA; 40S ribosomal protein sa; 40S ribosomal protein S3A; 40S ribosomal…
EC: 4.2.99.18, 2.7.11.1, 3.6.3.-, 3.1.-.-
UniProt: P08865, P61247, P15880, P62701, P23396, P62753, P62081, P62241, P46781, P46782, P62280, P46783, P62277, P62263, P25398, P62841, P08708, P62249, P62269, P39019, P63220, P62244, P62266, P62847, P60866, P62851, P42677, P62857, P62273, P62861, P62979, P63244, Q8N9N8, Q9BRS2, Q9ULX3, Q8N1G4
Ligands: ATP, ZN, MG
Pockets: 20 · best druggability 0.89

Structure

electron microscopy · 37 distinct chains · 28% helix, 21% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
2	Pre-18S ribosomal RNA	rna	1656	—	—
A	40S ribosomal protein sa	protein	216	37% helix, 11% strand	P08865
B	40S ribosomal protein S3A	protein	211	24% helix, 34% strand	P61247
C	40S ribosomal protein S2	protein	216	33% helix, 26% strand	P15880
E	40S ribosomal protein S4, X isoform	protein	255	11% helix, 31% strand	P62701
D	40S ribosomal protein S3 (EC 4.2.99.18)	protein	224	30% helix, 26% strand	P23396
G	40S ribosomal protein S6	protein	223	21% helix, 23% strand	P62753
H	40S ribosomal protein S7	protein	179	34% helix, 21% strand	P62081
I	40S ribosomal protein S8	protein	199	30% helix, 26% strand	P62241
J	40S ribosomal protein S9	protein	176	49% helix, 4% strand	P46781
F	40S ribosomal protein S5	protein	184	46% helix, 7% strand	P46782
L	40S ribosomal protein S11	protein	136	0% helix, 35% strand	P62280
K	40S ribosomal protein S10	protein	95	46% helix, 15% strand	P46783
N	40S ribosomal protein S13	protein	149	55% helix, 0% strand	P62277
O	40S ribosomal protein S14	protein	126	26% helix, 26% strand	P62263
M	40S ribosomal protein S12	protein	108	47% helix, 11% strand	P25398
P	40S ribosomal protein S15	protein	131	33% helix, 13% strand	P62841
R	40S ribosomal protein S17	protein	121	48% helix, 6% strand	P08708
Q	40S ribosomal protein S16	protein	140	39% helix, 20% strand	P62249
S	40S ribosomal protein S18	protein	141	38% helix, 7% strand	P62269
T	40S ribosomal protein S19	protein	141	50% helix, 10% strand	P39019
V	40S ribosomal protein S21	protein	83	18% helix, 27% strand	P63220
W	40S ribosomal protein S15A	protein	129	26% helix, 28% strand	P62244
X	40S ribosomal protein S23	protein	139	24% helix, 29% strand	P62266
Y	40S ribosomal protein S24	protein	122	34% helix, 28% strand	P62847
U	40S ribosomal protein S20	protein	98	28% helix, 47% strand	P60866
Z	40S ribosomal protein S25	protein	70	46% helix, 20% strand	P62851
b	40S ribosomal protein S27	protein	77	6% helix, 21% strand	P42677
c	40S ribosomal protein S28	protein	61	0% helix, 48% strand	P62857
d	40S ribosomal protein S29	protein	55	25% helix, 9% strand	P62273
e	40S ribosomal protein S30	protein	50	34% helix, 0% strand	P62861
f	Ubiquitin-40S ribosomal protein S27A	protein	57	0% helix, 18% strand	P62979
g	Receptor of activated protein C kinase 1	protein	295	0% helix, 52% strand	P63244
j	Probable RNA-binding protein EIF1AD	protein	115	19% helix, 30% strand	Q8N9N8
z	SERINE/THREONINE-protein kinase RIO1 (EC 2.7.11.1, 3.6.3.-)	protein	373	38% helix, 13% strand	Q9BRS2
y	RNA-binding protein NOB1 (EC 3.1.-.-)	protein	233	21% helix, 16% strand	Q9ULX3
k	Leucine-rich repeat-containing protein 47	protein	430	20% helix, 13% strand	Q8N1G4

Ligands and binding sites

ATP · Adenosine-5'-triphosphate · ligand · chains 2, z
ZN · Zinc ion · metal · 3 copies · chains d, f, y
MG · Magnesium ion · metal · 2 copies · chains z

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1085	0.89	0.91	126	empty
2	1142	0.89	0.91	96	empty
3	980	0.88	0.91	92	empty
4	1123	0.88	0.90	87	empty
5	875	0.86	0.89	79	empty

Interfaces

104 interfaces between chains.

2–G · 5245 Å² buried · 123 hydrogen bonds · protein-nucleic
2–I · 4322 Å² buried · 105 hydrogen bonds · protein-nucleic
2–z · 4315 Å² buried · 66 hydrogen bonds · protein-nucleic
2–E · 3889 Å² buried · 67 hydrogen bonds · protein-nucleic
2–X · 3697 Å² buried · 56 hydrogen bonds · protein-nucleic

Modifications

Observed in the structure: 1 disulfide (disulfide).

Compared structures

6ZXF · Cryo-EM structure of a late human pre-40S ribosomal subunit - State G · the same entry in another collection · chain I against I, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P08865
Every structure of UniProt P61247
Every structure of UniProt P15880
Every structure of UniProt P62701
Every structure of UniProt P23396
Every structure of UniProt P62753
Every structure of UniProt P62081
Every structure of UniProt P62241
Every structure of UniProt P46781
Every structure of UniProt P46782
Every structure of UniProt P62280
Every structure of UniProt P46783
Every structure of UniProt P62277
Every structure of UniProt P62263
Every structure of UniProt P25398
Every structure of UniProt P62841
Every structure of UniProt P08708
Every structure of UniProt P62249
Every structure of UniProt P62269
Every structure of UniProt P39019
Every structure of UniProt P63220
Every structure of UniProt P62244
Every structure of UniProt P62266
Every structure of UniProt P62847
Every structure of UniProt P60866
Every structure of UniProt P62851
Every structure of UniProt P42677
Every structure of UniProt P62857
Every structure of UniProt P62273
Every structure of UniProt P62861
Every structure of UniProt P62979
Every structure of UniProt P63244
Every structure of UniProt Q8N9N8
Every structure of UniProt Q9BRS2
Every structure of UniProt Q9ULX3
Every structure of UniProt Q8N1G4
Elsewhere: RCSB PDB 6ZXF · PDBe 6ZXF · UniProt P08865 · UniProt P61247 · UniProt P15880 · UniProt P62701 · UniProt P23396 · UniProt P62753 · UniProt P62081 · UniProt P62241 · UniProt P46781 · UniProt P46782 · UniProt P62280 · UniProt P46783 · UniProt P62277 · UniProt P62263 · UniProt P25398 · UniProt P62841 · UniProt P08708 · UniProt P62249 · UniProt P62269 · UniProt P39019 · UniProt P63220 · UniProt P62244 · UniProt P62266 · UniProt P62847 · UniProt P60866 · UniProt P62851 · UniProt P42677 · UniProt P62857 · UniProt P62273 · UniProt P62861 · UniProt P62979 · UniProt P63244 · UniProt Q8N9N8 · UniProt Q9BRS2 · UniProt Q9ULX3 · UniProt Q8N1G4

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