MolNFT v1 · #165153 · protein
Cryo-EM structure of the anaphase-promoting COMPLEX/CYCLOSOME, in complex with the mitotic checkpoint complex (APC/C-mcc) at 3.8 ångström resolution
6TLJ · Anaphase-promoting complex subunit 1: “Cryo-EM structure of the anaphase-promoting COMPLEX/CYCLOSOME, in complex with the mitotic checkpoint complex…”. Electron microscopy, 20 pockets (best druggability 0.88). MolNFT v1 #165153 on…
- PDB
- 6TLJ
- Type
- protein
- Length
- 9,200 residues
- Chains
- 23
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Anaphase-promoting complex subunit 1; Anaphase-promoting complex subunit 11; Cell…
- EC
- 2.7.11.1
- UniProt
- Q9H1A4, Q9NYG5, Q9UJX2, P60006, Q96DE5, P30260, Q8NHZ8, Q9UJX5, Q13042, Q9UM13, Q9BS18, Q9UJX6, Q9UJX4, Q12834, O60566, Q9UJX3, Q13257
- Pockets
- 20 · best druggability 0.88
Structure
electron microscopy · 21 distinct chains · 55% helix, 10% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Anaphase-promoting complex subunit 1 | protein | 1441 | 41% helix, 14% strand | Q9H1A4 |
| B | Anaphase-promoting complex subunit 11 | protein | 79 | 10% helix, 9% strand | Q9NYG5 |
| C | Cell division cycle protein 23 homolog | protein | 524 | 75% helix, 0% strand | Q9UJX2 |
| D | Anaphase-promoting complex subunit 15 | protein | 18 | 0% helix, 0% strand | P60006 |
| E | Anaphase-promoting complex subunit 16 | protein | 56 | 80% helix, 0% strand | Q96DE5 |
| F, H | Cell division cycle protein 27 homolog | protein | 483 | 76% helix, 0% strand | P30260 |
| G, W | Anaphase-promoting complex subunit CDC26 | protein | 25 | 44% helix, 0% strand | Q8NHZ8 |
| I | Anaphase-promoting complex subunit 4 | protein | 733 | 33% helix, 26% strand | Q9UJX5 |
| J | Cell division cycle protein 16 homolog | protein | 504 | 73% helix, 0% strand | Q13042 |
| K | Cell division cycle protein 16 homolog | protein | 493 | 77% helix, 0% strand | Q13042 |
| L | Anaphase-promoting complex subunit 10 | protein | 182 | 7% helix, 40% strand | Q9UM13 |
| M | Anaphase-promoting complex subunit 13 | protein | 59 | 22% helix, 0% strand | Q9BS18 |
| N | Anaphase-promoting complex subunit 2 | protein | 703 | 61% helix, 2% strand | Q9UJX6 |
| O | Anaphase-promoting complex subunit 5 | protein | 685 | 76% helix, 0% strand | Q9UJX4 |
| P | Cell division cycle protein 23 homolog | protein | 491 | 76% helix, 0% strand | Q9UJX2 |
| Q | Cell division cycle protein 20 homolog | protein | 365 | 0% helix, 44% strand | Q12834 |
| R | Cell division cycle protein 20 homolog | protein | 383 | 6% helix, 38% strand | Q12834 |
| S | Mitotic checkpoint SERINE/THREONINE-protein kinase BUB1 beta (EC 2.7.11.1) | protein | 293 | 45% helix, 2% strand | O60566 |
| X | Anaphase-promoting complex subunit 7 | protein | 484 | 80% helix, 0% strand | Q9UJX3 |
| Y | Anaphase-promoting complex subunit 7 | protein | 496 | 79% helix, 0% strand | Q9UJX3 |
| Z | Mitotic spindle assembly checkpoint protein MAD2A | protein | 195 | 35% helix, 35% strand | Q13257 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 849 | 0.88 | 0.90 | 91 | empty |
| 2 | 991 | 0.87 | 0.88 | 109 | empty |
| 3 | 1172 | 0.86 | 0.87 | 93 | empty |
| 4 | 627 | 0.85 | 0.89 | 71 | empty |
| 5 | 615 | 0.85 | 0.90 | 82 | empty |
Interfaces
60 interfaces between chains.
- A–O · 4536 Ų buried · 41 hydrogen bonds · protein-protein
- I–O · 3175 Ų buried · 26 hydrogen bonds · protein-protein
- F–H · 2986 Ų buried · 30 hydrogen bonds · protein-protein
- J–K · 2796 Ų buried · 47 hydrogen bonds · protein-protein
- X–Y · 2796 Ų buried · 23 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide (disulfide).
Compared structures
- 6TLJ · Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the… · the same entry in another collection · chain K against K, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9H1A4
- Every structure of UniProt Q9NYG5
- Every structure of UniProt Q9UJX2
- Every structure of UniProt P60006
- Every structure of UniProt Q96DE5
- Every structure of UniProt P30260
- Every structure of UniProt Q8NHZ8
- Every structure of UniProt Q9UJX5
- Every structure of UniProt Q13042
- Every structure of UniProt Q9UM13
- Every structure of UniProt Q9BS18
- Every structure of UniProt Q9UJX6
- Every structure of UniProt Q9UJX4
- Every structure of UniProt Q12834
- Every structure of UniProt O60566
- Every structure of UniProt Q9UJX3
- Every structure of UniProt Q13257
- Elsewhere: RCSB PDB 6TLJ · PDBe 6TLJ · UniProt Q9H1A4 · UniProt Q9NYG5 · UniProt Q9UJX2 · UniProt P60006 · UniProt Q96DE5 · UniProt P30260 · UniProt Q8NHZ8 · UniProt Q9UJX5 · UniProt Q13042 · UniProt Q9UM13 · UniProt Q9BS18 · UniProt Q9UJX6 · UniProt Q9UJX4 · UniProt Q12834 · UniProt O60566 · UniProt Q9UJX3 · UniProt Q13257
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