MolNFT v1 · #125576 · protein
Cryo-EM structure of the anaphase-promoting COMPLEX/CYCLOSOME, in complex with the mitotic checkpoint complex (APC/C-mcc) at 4.2 ångström resolution
5LCW · Anaphase-promoting complex subunit 1: “Cryo-EM structure of the anaphase-promoting COMPLEX/CYCLOSOME, in complex with the mitotic checkpoint complex…”. Electron microscopy, 20 pockets (best druggability 0.86). MolNFT v1 #125576 on…
- PDB
- 5LCW
- Type
- protein
- Length
- 9,173 residues
- Chains
- 23
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Anaphase-promoting complex subunit 1; Anaphase-promoting complex subunit 11; Cell…
- EC
- 2.7.11.1
- UniProt
- Q9H1A4, Q9NYG5, Q9UJX2, P60006, Q96DE5, P30260, Q8NHZ8, Q9UJX5, Q13042, Q9UM13, Q9BS18, Q9UJX6, Q9UJX4, Q12834, O60566, Q9UJX3, Q13257
- Pockets
- 20 · best druggability 0.86
Structure
electron microscopy · 21 distinct chains · 56% helix, 10% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Anaphase-promoting complex subunit 1 | protein | 1441 | 43% helix, 15% strand | Q9H1A4 |
| B | Anaphase-promoting complex subunit 11 | protein | 79 | 10% helix, 15% strand | Q9NYG5 |
| C | Cell division cycle protein 23 homolog | protein | 524 | 76% helix, 0% strand | Q9UJX2 |
| D | Anaphase-promoting complex subunit 15 | protein | 18 | 0% helix, 0% strand | P60006 |
| E | Anaphase-promoting complex subunit 16 | protein | 56 | 82% helix, 0% strand | Q96DE5 |
| F, H | Cell division cycle protein 27 homolog | protein | 483 | 77% helix, 0% strand | P30260 |
| G, W | Anaphase-promoting complex subunit CDC26 | protein | 25 | 48% helix, 0% strand | Q8NHZ8 |
| I | Anaphase-promoting complex subunit 4 | protein | 733 | 33% helix, 24% strand | Q9UJX5 |
| J | Cell division cycle protein 16 homolog | protein | 504 | 75% helix, 0% strand | Q13042 |
| K | Cell division cycle protein 16 homolog | protein | 493 | 79% helix, 0% strand | Q13042 |
| L | Anaphase-promoting complex subunit 10 | protein | 182 | 8% helix, 37% strand | Q9UM13 |
| M | Anaphase-promoting complex subunit 13 | protein | 59 | 22% helix, 0% strand | Q9BS18 |
| N | Anaphase-promoting complex subunit 2 | protein | 703 | 60% helix, 2% strand | Q9UJX6 |
| O | Anaphase-promoting complex subunit 5 | protein | 685 | 77% helix, 0% strand | Q9UJX4 |
| P | Cell division cycle protein 23 homolog | protein | 491 | 77% helix, 0% strand | Q9UJX2 |
| Q | Cell division cycle protein 20 homolog | protein | 354 | 1% helix, 46% strand | Q12834 |
| R | Cell division cycle protein 20 homolog | protein | 383 | 7% helix, 41% strand | Q12834 |
| S | Mitotic checkpoint SERINE/THREONINE-protein kinase BUB1 BETA,MITOTIC checkpoint SERINE/THREONINE-protein kinase BUB1 beta (EC 2.7.11.1) | protein | 277 | 49% helix, 1% strand | O60566 |
| X | Anaphase-promoting complex subunit 7 | protein | 484 | 80% helix, 0% strand | Q9UJX3 |
| Y | Anaphase-promoting complex subunit 7 | protein | 496 | 80% helix, 0% strand | Q9UJX3 |
| Z | Mitotic spindle assembly checkpoint protein MAD2A | protein | 195 | 33% helix, 35% strand | Q13257 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1176 | 0.86 | 0.87 | 89 | empty |
| 2 | 790 | 0.86 | 0.89 | 72 | empty |
| 3 | 829 | 0.86 | 0.88 | 80 | empty |
| 4 | 569 | 0.84 | 0.90 | 84 | empty |
| 5 | 942 | 0.84 | 0.86 | 108 | empty |
Interfaces
61 interfaces between chains.
- A–O · 4761 Ų buried · 34 hydrogen bonds · protein-protein
- I–O · 3275 Ų buried · 22 hydrogen bonds · protein-protein
- F–H · 3115 Ų buried · 20 hydrogen bonds · protein-protein
- X–Y · 2874 Ų buried · 13 hydrogen bonds · protein-protein
- J–K · 2844 Ų buried · 36 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide, 1 isopeptide (disulfide, isopeptide).
Compared structures
- 5LCW · Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the… · the same entry in another collection · chain J against J, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9H1A4
- Every structure of UniProt Q9NYG5
- Every structure of UniProt Q9UJX2
- Every structure of UniProt P60006
- Every structure of UniProt Q96DE5
- Every structure of UniProt P30260
- Every structure of UniProt Q8NHZ8
- Every structure of UniProt Q9UJX5
- Every structure of UniProt Q13042
- Every structure of UniProt Q9UM13
- Every structure of UniProt Q9BS18
- Every structure of UniProt Q9UJX6
- Every structure of UniProt Q9UJX4
- Every structure of UniProt Q12834
- Every structure of UniProt O60566
- Every structure of UniProt Q9UJX3
- Every structure of UniProt Q13257
- Elsewhere: RCSB PDB 5LCW · PDBe 5LCW · UniProt Q9H1A4 · UniProt Q9NYG5 · UniProt Q9UJX2 · UniProt P60006 · UniProt Q96DE5 · UniProt P30260 · UniProt Q8NHZ8 · UniProt Q9UJX5 · UniProt Q13042 · UniProt Q9UM13 · UniProt Q9BS18 · UniProt Q9UJX6 · UniProt Q9UJX4 · UniProt Q12834 · UniProt O60566 · UniProt Q9UJX3 · UniProt Q13257
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