Research workbench
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human gipr-gs complex
Analyse Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7FIN
- Type
- protein
- Length
- 1,169 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Gastric inhibitory polypeptide RECEPTOR,HUMAN glucose- dependent insulinotropic…
- UniProt
- P48546, P04896, P54311, P63212
- Ligands
- CLR, GGL
- Pockets
- 13 · best druggability 0.83
Structure
electron microscopy · 6 distinct chains · 39% helix, 24% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| R | Gastric inhibitory polypeptide RECEPTOR,HUMAN glucose- dependent insulinotropic polypeptide receptor | protein | 380 | 67% helix, 6% strand | P48546 |
| P | Peptide 20 | protein | 30 | 90% helix, 0% strand | — |
| A | Guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 232 | 48% helix, 17% strand | P04896 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 341 | 7% helix, 48% strand | P54311 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 57 | 54% helix, 0% strand | P63212 |
| N | Nanobody-35 | protein | 129 | 5% helix, 43% strand | — |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
13 pockets found on a 1 Å grid, 9 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 809 | 0.83 | 0.86 | 73 | empty |
| 2 | 752 | 0.77 | 0.82 | 75 | empty |
| 3 | 924 | 0.75 | 0.79 | 67 | empty |
| 4 | 531 | 0.74 | 0.82 | 64 | empty |
| 5 | 348 | 0.69 | 0.82 | 46 | GGL |
Interfaces
7 interfaces between chains.
- B–G · 2329 Ų buried · 15 hydrogen bonds · protein-protein
- R–P · 1869 Ų buried · 13 hydrogen bonds · protein-protein
- A–B · 1514 Ų buried · 23 hydrogen bonds · protein-protein
- R–A · 1174 Ų buried · 9 hydrogen bonds · protein-protein
- A–N · 1023 Ų buried · 8 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 6 disulfide, 1 covalent, 1 modified (disulfide, covalent ligand, nonstandard).
Compared structures
- 7FIN · Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GIPR-Gs… · the same entry in another collection · chain N against N, TM-score 1.00
- P54311 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P04896 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(S) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.84
- P48546 · AlphaFold monomer V2.0 prediction for gastric inhibitory polypeptide receptor (P48546) · a predicted model of the same protein · chain R against A, TM-score 0.79
- P63212 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.67
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P48546
- Every structure of UniProt P04896
- Every structure of UniProt P54311
- Every structure of UniProt P63212
- Elsewhere: RCSB PDB 7FIN · PDBe 7FIN · UniProt P48546 · UniProt P04896 · UniProt P54311 · UniProt P63212
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