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Crystal structure of tubulin-stathmin-ttl-tubulysin M complex

Analyse Crystal structure of tubulin-stathmin-ttl-tubulysin M complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 4ZOL
Type: protein
Length: 2,206 residues
Chains: 6
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Tubulin alpha-1B chain; Tubulin beta chain; Stathmin-4; Tubulin-tyrosine ligase
UniProt: Q2XVP4, P02554, P63043, E1BQ43
Ligands: GTP, GDP, 55Q, ACP, MG, CA
Pockets: 20 · best druggability 0.84

Structure

x-ray diffraction · 6 distinct chains · 46% helix, 18% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Tubulin alpha-1B chain	protein	439	45% helix, 18% strand	Q2XVP4
B	Tubulin beta chain	protein	430	49% helix, 19% strand	P02554
C	Tubulin alpha-1B chain	protein	440	46% helix, 17% strand	Q2XVP4
D	Tubulin beta chain	protein	427	48% helix, 19% strand	P02554
E	Stathmin-4	protein	122	75% helix, 13% strand	P63043
F	Tubulin-tyrosine ligase	protein	348	31% helix, 21% strand	E1BQ43

Ligands and binding sites

GTP · Guanosine-5'-triphosphate · ligand · 2 copies · chains A, B, C, D
GDP · Guanosine-5'-diphosphate · ligand · 2 copies · chains B, D
55Q · (2R,4R)-4-{[(2-{(1R,3R)-1-(acetyloxy)-4-methyl-3-[methyl(N-{[(2S)-1-methylpiperidin-2-yl]carbonyl}-D-isoleucyl)amino]pentyl}-1,3-thiazol-4-yl)carbonyl]amino}-2-methyl-5-phenylpentanoic acid · ligand · 2 copies · chains B, C, D
ACP · Phosphomethylphosphonic acid adenylate ester · ligand · chains F
MG · Magnesium ion · metal · 4 copies · chains A, B, C, D
CA · Calcium ion · metal · 2 copies · chains A, C
GOL · Glycerol · additive · 7 copies · chains A, E, B, C, F
MES · 2-(N-morpholino)-ethanesulfonic acid · additive · 2 copies · chains B, A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 17 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	844	0.84	0.86	105	GTP, MG, GOL
2	805	0.82	0.85	103	GDP, MG, 55Q
3	194	0.81	0.95	41	empty
4	417	0.77	0.86	55	GTP, MG
5	857	0.77	0.80	65	empty

Interfaces

9 interfaces between chains.

A–E · 1644 Å² buried · 24 hydrogen bonds · protein-protein
A–B · 1423 Å² buried · 17 hydrogen bonds · protein-protein
C–D · 1402 Å² buried · 16 hydrogen bonds · protein-protein
B–C · 967 Å² buried · 18 hydrogen bonds · protein-protein
A–F · 836 Å² buried · 9 hydrogen bonds · protein-protein

Compared structures

4ZOL · Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex · the same entry in another collection · chain F against F, TM-score 1.00
P02554 · AlphaFold monomer V2.0 prediction for tubulin beta chain (P02554) · a predicted model of the same protein · chain D against A, TM-score 0.98
Q2XVP4 · AlphaFold monomer V2.0 prediction for tubulin alpha-1B chain (Q2XVP4) · a predicted model of the same protein · chain C against A, TM-score 0.96
P63043 · AlphaFold monomer V2.0 prediction for stathmin-4 (P63043) · a predicted model of the same protein · chain E against A, TM-score 0.77

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q2XVP4
Every structure of UniProt P02554
Every structure of UniProt P63043
Every structure of UniProt E1BQ43
Elsewhere: RCSB PDB 4ZOL · PDBe 4ZOL · UniProt Q2XVP4 · UniProt P02554 · UniProt P63043 · UniProt E1BQ43

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