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MolNFT v1 · #148951 · protein + DNA + RNA

Cryoem structure of E.COLI RNA polymerase paused elongation complex bound to nusa

6FLQ: “Cryoem structure of E.COLI RNA polymerase paused elongation complex bound to nusa”. Electron microscopy, 2 ligands (ZN, MG). MolNFT v1 #148951 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB
6FLQ
Type
protein + DNA + RNA
Length
3,959 residues
Chains
9
Method
electron microscopy
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
UniProt
P0A7Z4, P0A8V2, P0A8T7, P0A800, P0AFF6
Ligands
ZN, MG

Structure

electron microscopy · 9 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—protein310—P0A7Z4
B—protein298—P0A7Z4
C—protein1341—P0A8V2
D—protein1334—P0A8T7
E—protein90—P0A800
F—protein495—P0AFF6
N—dna31——
R—rna21——
T—dna39——

Ligands and binding sites

  • ZN · Zinc ion · metal · 2 copies · chains D
  • MG · Magnesium ion · metal · chains D, R

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6FLQ · CryoEM structure of E.coli RNA polymerase paused elongation complex bound to NusA · the same entry in another collection · chain T against T, TM-score 1.00
  • P0A8V2 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta (P0A8V2) · a predicted model of the same protein · chain C against A, TM-score 0.95
  • P0AFF6 · AlphaFold monomer V2.0 prediction for transcription TERMINATION/ANTITERMINATION protein… · a predicted model of the same protein · chain F against A, TM-score 0.76

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