MolNFT v1 · #119749 · protein
RNA polymerase I-RRN3 complex at 4.8 a resolution
5G5L · DNA-directed RNA polymerase I subunit RPA190: “RNA polymerase I-RRN3 complex at 4.8 a resolution”. Electron microscopy, 1 ligand (ZN), 20 pockets (best druggability 0.83). MolNFT v1 #119749 on GenesisL1: 3D structure, binding sites…
- PDB
- 5G5L
- Type
- protein
- Length
- 4,686 residues
- Chains
- 15
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA-directed RNA polymerase I subunit RPA190; DNA-directed RNA polymerase I subunit…
- EC
- 2.7.7.6
- UniProt
- P10964, P22138, P07703, P50106, P20434, P20435, P46669, P20436, P32529, P22139, P28000, P40422, Q01080, P47006, P36070
- Ligands
- ZN
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 15 distinct chains · 36% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA-directed RNA polymerase I subunit RPA190 (EC 2.7.7.6) | protein | 1480 | 44% helix, 14% strand | P10964 |
| B | DNA-directed RNA polymerase I subunit RPA135 (EC 2.7.7.6) | protein | 1174 | 29% helix, 25% strand | P22138 |
| C | DNA-directed RNA polymerases I and III subunit RPAC1 | protein | 305 | 28% helix, 32% strand | P07703 |
| D | DNA-directed RNA polymerase I subunit RPA14 | protein | 58 | 52% helix, 10% strand | P50106 |
| E | DNA-directed RNA polymerases I, II, and III subunit rpabc 1 | protein | 212 | 40% helix, 23% strand | P20434 |
| F | DNA-directed RNA polymerases I, II, and III subunit rpabc 2 | protein | 100 | 43% helix, 13% strand | P20435 |
| G | DNA-directed RNA polymerase I subunit RPA43 | protein | 193 | 8% helix, 44% strand | P46669 |
| H | DNA-directed RNA polymerases I, II, and III subunit rpabc 3 | protein | 131 | 3% helix, 53% strand | P20436 |
| I | DNA-directed RNA polymerase I subunit RPA12 | protein | 107 | 9% helix, 28% strand | P32529 |
| J | DNA-directed RNA polymerases I, II, and III subunit rpabc 5 | protein | 69 | 41% helix, 4% strand | P22139 |
| K | DNA-directed RNA polymerases I and III subunit RPAC2 | protein | 101 | 40% helix, 25% strand | P28000 |
| L | DNA-directed RNA polymerases I, II, and III subunit rpabc 4 | protein | 43 | 0% helix, 28% strand | P40422 |
| M | DNA-directed RNA polymerase I subunit RPA49 | protein | 105 | 7% helix, 55% strand | Q01080 |
| N | DNA-directed RNA polymerase I subunit RPA34 | protein | 145 | 8% helix, 27% strand | P47006 |
| O | RNA polymerase I-specific transcription initiation factor RRN3 | protein | 463 | 70% helix, 0% strand | P36070 |
Ligands and binding sites
- ZN · Zinc ion · metal · 7 copies · chains A, B, I, J, L
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 701 | 0.83 | 0.87 | 96 | empty |
| 2 | 508 | 0.83 | 0.89 | 57 | empty |
| 3 | 924 | 0.82 | 0.84 | 99 | empty |
| 4 | 1063 | 0.82 | 0.83 | 118 | empty |
| 5 | 740 | 0.82 | 0.86 | 81 | empty |
Interfaces
37 interfaces between chains.
- A–B · 9419 Ų buried · 119 hydrogen bonds · protein-protein
- A–E · 2628 Ų buried · 28 hydrogen bonds · protein-protein
- M–N · 2474 Ų buried · 34 hydrogen bonds · protein-protein
- A–I · 2273 Ų buried · 35 hydrogen bonds · protein-protein
- C–K · 2107 Ų buried · 14 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide, 1 isopeptide (disulfide, isopeptide).
Compared structures
- 5G5L · RNA polymerase I-Rrn3 complex at 4.8 A resolution · the same entry in another collection · chain J against J, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P10964
- Every structure of UniProt P22138
- Every structure of UniProt P07703
- Every structure of UniProt P50106
- Every structure of UniProt P20434
- Every structure of UniProt P20435
- Every structure of UniProt P46669
- Every structure of UniProt P20436
- Every structure of UniProt P32529
- Every structure of UniProt P22139
- Every structure of UniProt P28000
- Every structure of UniProt P40422
- Every structure of UniProt Q01080
- Every structure of UniProt P47006
- Every structure of UniProt P36070
- Elsewhere: RCSB PDB 5G5L · PDBe 5G5L · UniProt P10964 · UniProt P22138 · UniProt P07703 · UniProt P50106 · UniProt P20434 · UniProt P20435 · UniProt P46669 · UniProt P20436 · UniProt P32529 · UniProt P22139 · UniProt P28000 · UniProt P40422 · UniProt Q01080 · UniProt P47006 · UniProt P36070
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.