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Cryoem structure of crosslinked E.COLI RNA polymerase elongation complex

Analyse Cryoem structure of crosslinked E.COLI RNA polymerase elongation complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB
6ALF
Type
protein + DNA + RNA
Length
3,237 residues
Chains
8
Method
electron microscopy
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
UniProt
P0A7Z4, P0A8V2, P0A8T7, P0A800
Ligands
ZN, MG

Structure

electron microscopy · 8 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—dna22——
B—dna29——
R—rna12——
G—protein222—P0A7Z4
H—protein220—P0A7Z4
I—protein1316—P0A8V2
J—protein1337—P0A8T7
K—protein79—P0A800

Ligands and binding sites

  • ZN · Zinc ion · metal · 2 copies · chains J
  • MG · Magnesium ion · metal · chains R, J

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6ALF · CryoEM structure of crosslinked E.coli RNA polymerase elongation complex · the same entry in another collection · chain K against K, TM-score 1.00
  • P0A8V2 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta (P0A8V2) · a predicted model of the same protein · chain I against A, TM-score 0.99
  • P0A8T7 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta' (P0A8T7) · a predicted model of the same protein · chain J against A, TM-score 0.93

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