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MolNFT v2 · #206425 · protein + RNA

Human mitochondrial small subunit assembly intermediate (State E)

8CST: “Human mitochondrial small subunit assembly intermediate (State E)”. Electron microscopy at 2.85, 8 ligands (FES, SF4, NAD). MolNFT v2 #206425 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB
8CST
Type
protein + RNA
Length
6,699 residues
Chains
32
Method
electron microscopy
Collection
MolNFT v2 / PDB Bank
Contract
0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
Owner
0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
Resolution
2.85
Source
Homo sapiens
UniProt
P82930, P82673, Q9NWT8, Q96EY7, Q8N0V3, Q9H7H0, Q9Y399, Q96EL2, P82675, P82932, Q9Y2R9, P82933, P82664, P82912, O15235, O60783, P82914, Q9Y3D3, Q9Y2R5, Q9Y676, Q9Y3D5, P82921, P82650, Q9Y3D9, P82663, Q9BYN8, Q92552, Q9Y2Q9, P51398, Q92665, Q9Y291
Ligands
FES, SF4, NAD, ATP, GDP, MG, K, ZN

Structure

electron microscopy · resolution 2.85 · 32 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
0—protein211—P82930
1—protein259—P82673
3—protein70—Q9NWT8
4—protein566—Q96EY7
6—protein126—Q8N0V3
7—protein401—Q9H7H0
A—rna888——
B—protein220—Q9Y399
C—protein126—Q96EL2
D—protein232—P82675
E—protein108—P82932
F—protein206—Q9Y2R9
G—protein292—P82933
H—protein130—P82664
I—protein137—P82912
J—protein107—O15235
K—protein100—O60783
L—protein161—P82914
M—protein115—Q9Y3D3
N—protein109—Q9Y2R5
O—protein190—Q9Y676
P—protein96—Q9Y3D5
Q—protein86—P82921
R—protein291—P82650
S—protein133—Q9Y3D9
T—protein164—P82663
U—protein174—Q9BYN8
V—protein359—Q92552
W—protein98—Q9Y2Q9
X—protein352—P51398
Y—protein123—Q92665
Z—protein69—Q9Y291

Ligands and binding sites

  • FES · Fe2/s2 (inorganic) cluster · ligand · 2 copies · chains E, P, M, T
  • SF4 · Iron/sulfur cluster · ligand · chains 7
  • NAD · Nicotinamide-adenine-dinucleotide · ligand · chains A, J, L
  • ATP · Adenosine-5'-triphosphate · ligand · chains X
  • GDP · Guanosine-5'-diphosphate · ligand · chains X
  • MG · Magnesium ion · metal · 30 copies · chains A, B, W, X
  • K · Potassium ion · metal · 4 copies · chains A
  • ZN · Zinc ion · metal · chains O

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • Q96EY7 · AlphaFold monomer V2.0 prediction for pentatricopeptide repeat domain-containing protein… · a predicted model of the same protein · chain 4 against A, TM-score 0.99
  • P82650 · AlphaFold monomer V2.0 prediction for 28S ribosomal protein S22, mitochondrial (P82650) · a predicted model of the same protein · chain R against A, TM-score 0.99
  • Q9Y2R5 · AlphaFold monomer V2.0 prediction for 28S ribosomal protein S17, mitochondrial (Q9Y2R5) · a predicted model of the same protein · chain N against A, TM-score 0.97
  • Q9H7H0 · AlphaFold monomer V2.0 prediction for methyltransferase-like protein 17, mitochondrial… · a predicted model of the same protein · chain 7 against A, TM-score 0.96
  • Q9NWT8 · AlphaFold monomer V2.0 prediction for aurora kinase a-interacting protein (Q9NWT8) · a predicted model of the same protein · chain 3 against A, TM-score 0.96

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