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MolNFT v2 · #145761 · protein + DNA + RNA

CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH

6C6S: “CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH”. Electron microscopy at 3.7, 2 ligands (ZN, MG). MolNFT v2 #145761 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB
6C6S
Type
protein + DNA + RNA
Length
3,428 residues
Chains
9
Method
electron microscopy
Collection
MolNFT v2 / PDB Bank
Contract
0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
Owner
0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
Resolution
3.7
Source
Escherichia coli; Escherichia coli (strain K12); SYNTHETIC CONSTRUCT
UniProt
P0A7Z4, P0A8V2, P0A8T7, P0A800, P0AFW0
Ligands
ZN, MG

Structure

electron microscopy · resolution 3.7 · 9 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—dna29——
B—dna29——
R—rna10——
G—protein221—P0A7Z4
H—protein219—P0A7Z4
I—protein1341—P0A8V2
J—protein1335—P0A8T7
K—protein83—P0A800
D—protein161—P0AFW0

Ligands and binding sites

  • ZN · Zinc ion · metal · 2 copies · chains J
  • MG · Magnesium ion · metal · chains R, J

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6C6S · Cryoem structure of E.COLI RNA polymerase elongation complex bound with rfah · the same entry in another collection · chain R against R, TM-score 1.00
  • P0A8V2 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta (P0A8V2) · a predicted model of the same protein · chain I against A, TM-score 0.98
  • P0A8T7 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta' (P0A8T7) · a predicted model of the same protein · chain J against A, TM-score 0.94

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