MolNFT v1 · #187849 · protein + RNA

Structure of the wt ires EIF5B-containing pre-48S initiation complex, open conformation. structure 14(WT)

7SYV · 18S rRNA: “Structure of the wt ires EIF5B-containing pre-48S initiation complex, open conformation. structure 14(WT)”. Electron microscopy, 4 ligands (GTP, ZN, MG), 20 pockets (best druggability 0.88). MolNFT v1 #187849 on…

PDB: 7SYV
Type: protein + RNA
Length: 7,638 residues
Chains: 39
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: 18S rRNA; 40S ribosomal protein S24; Eukaryotic translation initiation factor 1A…
UniProt: A0A5F9CNN4, P47813, G1TDB3, G1TLT8, G1TFE8, G1SS70, G1TZ76, G1TUT9, G1TIB4, G1TNM3, G1U7M4, G1TK17, G1T8A2, G1TFM5, G1SK22, G1TM55, G1SJB4, G1SVB0, G1TJW1, A0A087WNH4, B7NZS8, G1TRL5, G1TPV3, G1TRM4, G1SFR8, G1SP51, G1U472, G1U0Q2, G1SGX4, G1TU13, G1TPG3, G1TN62, G1SIZ2, G1TM82, G1TG89, G1SZ47
Ligands: GTP, ZN, MG, NA
Pockets: 20 · best druggability 0.88

Structure

electron microscopy · 39 distinct chains · 28% helix, 21% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
2	18S rRNA	rna	1680	—	—
Z	40S ribosomal protein S24	protein	124	34% helix, 29% strand	A0A5F9CNN4
A	Eukaryotic translation initiation factor 1A, X-chromosomal	protein	102	9% helix, 31% strand	P47813
a	40S ribosomal protein S25	protein	77	45% helix, 17% strand	G1TDB3
B	40S ribosomal protein sa	protein	217	38% helix, 10% strand	G1TLT8
b	ES26	protein	101	11% helix, 23% strand	G1TFE8
C	ES1	protein	213	23% helix, 29% strand	G1SS70
c	ES27	protein	83	7% helix, 22% strand	G1TZ76
D	40S ribosomal protein S2	protein	221	29% helix, 24% strand	G1TUT9
d	ES28	protein	67	0% helix, 39% strand	G1TIB4
E	US3	protein	228	29% helix, 25% strand	G1TNM3
e	ES29	protein	55	31% helix, 9% strand	G1U7M4
F	40S ribosomal protein S4	protein	262	15% helix, 32% strand	G1TK17
f	ES30	protein	57	26% helix, 0% strand	G1T8A2
G	US7	protein	191	46% helix, 5% strand	G1TFM5
g	40S ribosomal protein S27A	protein	68	6% helix, 6% strand	G1SK22
H	ES6	protein	237	27% helix, 20% strand	G1TM55
h	RACK1	protein	313	0% helix, 47% strand	G1SJB4
I	40S ribosomal protein S7	protein	185	33% helix, 19% strand	G1SVB0
i	Met-tRNA-I-met	rna	75	—	—
J	ES8	protein	206	28% helix, 24% strand	G1TJW1
n	60S ribosomal protein L41	protein	25	88% helix, 0% strand	A0A087WNH4
K	US4	protein	185	52% helix, 5% strand	B7NZS8
x	Eukaryotic translation initiation factor 5B	protein	627	29% helix, 21% strand	G1TRL5
L	ES10	protein	96	42% helix, 9% strand	G1TPV3
z	HCV ires	rna	258	—	—
M	US17	protein	151	3% helix, 28% strand	G1TRM4
N	ES12	protein	117	40% helix, 9% strand	G1SFR8
O	US15	protein	149	50% helix, 0% strand	G1SP51
P	US11	protein	136	24% helix, 23% strand	G1U472
Q	US19	protein	120	30% helix, 15% strand	G1U0Q2
R	US9	protein	142	42% helix, 17% strand	G1SGX4
S	ES17	protein	132	45% helix, 3% strand	G1TU13
T	US13	protein	144	40% helix, 6% strand	G1TPG3
U	ES19	protein	141	50% helix, 10% strand	G1TN62
V	US10	protein	100	26% helix, 36% strand	G1SIZ2
W	40S ribosomal protein S21	protein	83	20% helix, 27% strand	G1TM82
X	US8	protein	129	31% helix, 22% strand	G1TG89
Y	US12	protein	141	21% helix, 29% strand	G1SZ47

Ligands and binding sites

GTP · Guanosine-5'-triphosphate · ligand · chains x
ZN · Zinc ion · metal · 2 copies · chains b, g
MG · Magnesium ion · metal · chains x
NA · Sodium ion · metal · chains x

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	953	0.88	0.91	91	empty
2	1062	0.88	0.90	80	empty
3	705	0.88	0.92	88	empty
4	819	0.87	0.91	82	empty
5	522	0.87	0.94	70	empty

Interfaces

113 interfaces between chains.

2–H · 5294 Å² buried · 97 hydrogen bonds · protein-nucleic
2–J · 4231 Å² buried · 82 hydrogen bonds · protein-nucleic
2–F · 3716 Å² buried · 54 hydrogen bonds · protein-nucleic
2–Y · 3545 Å² buried · 71 hydrogen bonds · protein-nucleic
2–K · 3504 Å² buried · 55 hydrogen bonds · protein-nucleic

Modifications

Observed in the structure: 4 disulfide, 1 covalent (disulfide, covalent ligand).

Compared structures

7SYV · Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation.… · the same entry in another collection · chain 2 against 2, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt A0A5F9CNN4
Every structure of UniProt P47813
Every structure of UniProt G1TDB3
Every structure of UniProt G1TLT8
Every structure of UniProt G1TFE8
Every structure of UniProt G1SS70
Every structure of UniProt G1TZ76
Every structure of UniProt G1TUT9
Every structure of UniProt G1TIB4
Every structure of UniProt G1TNM3
Every structure of UniProt G1U7M4
Every structure of UniProt G1TK17
Every structure of UniProt G1T8A2
Every structure of UniProt G1TFM5
Every structure of UniProt G1SK22
Every structure of UniProt G1TM55
Every structure of UniProt G1SJB4
Every structure of UniProt G1SVB0
Every structure of UniProt G1TJW1
Every structure of UniProt A0A087WNH4
Every structure of UniProt B7NZS8
Every structure of UniProt G1TRL5
Every structure of UniProt G1TPV3
Every structure of UniProt G1TRM4
Every structure of UniProt G1SFR8
Every structure of UniProt G1SP51
Every structure of UniProt G1U472
Every structure of UniProt G1U0Q2
Every structure of UniProt G1SGX4
Every structure of UniProt G1TU13
Every structure of UniProt G1TPG3
Every structure of UniProt G1TN62
Every structure of UniProt G1SIZ2
Every structure of UniProt G1TM82
Every structure of UniProt G1TG89
Every structure of UniProt G1SZ47
Elsewhere: RCSB PDB 7SYV · PDBe 7SYV · UniProt A0A5F9CNN4 · UniProt P47813 · UniProt G1TDB3 · UniProt G1TLT8 · UniProt G1TFE8 · UniProt G1SS70 · UniProt G1TZ76 · UniProt G1TUT9 · UniProt G1TIB4 · UniProt G1TNM3 · UniProt G1U7M4 · UniProt G1TK17 · UniProt G1T8A2 · UniProt G1TFM5 · UniProt G1SK22 · UniProt G1TM55 · UniProt G1SJB4 · UniProt G1SVB0 · UniProt G1TJW1 · UniProt A0A087WNH4 · UniProt B7NZS8 · UniProt G1TRL5 · UniProt G1TPV3 · UniProt G1TRM4 · UniProt G1SFR8 · UniProt G1SP51 · UniProt G1U472 · UniProt G1U0Q2 · UniProt G1SGX4 · UniProt G1TU13 · UniProt G1TPG3 · UniProt G1TN62 · UniProt G1SIZ2 · UniProt G1TM82 · UniProt G1TG89 · UniProt G1SZ47

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