MolNFT v1 · #186319 · protein

Cryo-EM of human gastric inhibitory polypeptide receptor gipr bound to gip

7RA3 · Guanine nucleotide-binding protein G(I) subunit alpha-3…: “Cryo-EM of human gastric inhibitory polypeptide receptor gipr bound to gip”. Electron microscopy, 17 pockets (best druggability 0.83). MolNFT v1 #186319 on GenesisL1: 3D…

PDB: 7RA3
Type: protein
Length: 1,381 residues
Chains: 7
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Guanine nucleotide-binding protein G(I) subunit alpha-3, isoform gnas-2 of guanine…
UniProt: P08754, P63092, P62873, P59768, P09681, P48546
Pockets: 17 · best druggability 0.83

Structure

electron microscopy · 7 distinct chains · 33% helix, 26% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Guanine nucleotide-binding protein G(I) subunit alpha-3, isoform gnas-2 of guanine nucleotide-binding protein G(S) subunit alpha isoforms short	protein	220	45% helix, 15% strand	P08754, P63092
B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	protein	339	7% helix, 45% strand	P62873
E	Single-chain variable fragment 16	protein	232	5% helix, 48% strand	—
G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	protein	59	58% helix, 0% strand	P59768
N	Nanobody 35	protein	128	7% helix, 39% strand	—
P	Gastric inhibitory polypeptide	protein	32	94% helix, 0% strand	P09681
R	Gastric inhibitory polypeptide receptor	protein	371	66% helix, 2% strand	P48546

Pockets

17 pockets found on a 1 Å grid, 12 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	756	0.83	0.86	60	empty
2	840	0.75	0.79	61	empty
3	995	0.74	0.77	89	empty
4	444	0.68	0.79	66	empty
5	139	0.66	0.88	22	empty

Interfaces

9 interfaces between chains.

B–G · 2068 Å² buried · 18 hydrogen bonds · protein-protein
P–R · 1898 Å² buried · 21 hydrogen bonds · protein-protein
A–B · 1418 Å² buried · 19 hydrogen bonds · protein-protein
A–R · 1048 Å² buried · 12 hydrogen bonds · protein-protein
A–N · 807 Å² buried · 13 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 8 disulfide (disulfide).

Compared structures

7RA3 · cryo-EM of human Gastric inhibitory polypeptide receptor GIPR bound to GIP · the same entry in another collection · chain R against R, TM-score 1.00
P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.90
P09681 · AlphaFold monomer V2.0 prediction for gastric inhibitory polypeptide (P09681) · a predicted model of the same protein · chain P against A, TM-score 0.80
P48546 · AlphaFold monomer V2.0 prediction for gastric inhibitory polypeptide receptor (P48546) · a predicted model of the same protein · chain R against A, TM-score 0.78
P08754 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.75

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P08754
Every structure of UniProt P63092
Every structure of UniProt P62873
Every structure of UniProt P59768
Every structure of UniProt P09681
Every structure of UniProt P48546
Elsewhere: RCSB PDB 7RA3 · PDBe 7RA3 · UniProt P08754 · UniProt P63092 · UniProt P62873 · UniProt P59768 · UniProt P09681 · UniProt P48546

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