MolNFT v1 · #181464 · protein
KIF14[391-743] - AMP-pnp closed state class in complex with a microtubule
7LVQ · Tubulin alpha-1B chain: “KIF14[391-743] - AMP-pnp closed state class in complex with a microtubule”. Electron microscopy, 5 ligands (GTP, GDP, TA1), 16 pockets (best druggability 0.84). MolNFT v1 #181464 on GenesisL1: 3D structure…
- PDB
- 7LVQ
- Type
- protein
- Length
- 1,226 residues
- Chains
- 3
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Tubulin alpha-1B chain; Tubulin beta-2B chain; Kinesin-like protein KIF14
- UniProt
- Q2XVP4, A0A287AGU7, L0N7N1
- Ligands
- GTP, GDP, TA1, ANP, MG
- Pockets
- 16 · best druggability 0.84
Structure
electron microscopy · 3 distinct chains · 39% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Tubulin alpha-1B chain | protein | 441 | 41% helix, 16% strand | Q2XVP4 |
| B | Tubulin beta-2B chain | protein | 429 | 43% helix, 19% strand | A0A287AGU7 |
| K | Kinesin-like protein KIF14 | protein | 356 | 32% helix, 28% strand | L0N7N1 |
Ligands and binding sites
- GTP · Guanosine-5'-triphosphate · ligand · chains A, B
- GDP · Guanosine-5'-diphosphate · ligand · chains B
- TA1 · Taxol · ligand · chains B
- ANP · Phosphoaminophosphonic acid-adenylate ester · ligand · chains K
- MG · Magnesium ion · metal · 2 copies · chains A, K
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
16 pockets found on a 1 Å grid, 9 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 263 | 0.84 | 0.96 | 44 | GTP, MG |
| 2 | 1007 | 0.81 | 0.83 | 83 | empty |
| 3 | 65 | 0.70 | 0.96 | 25 | empty |
| 4 | 372 | 0.69 | 0.82 | 46 | empty |
| 5 | 492 | 0.65 | 0.77 | 64 | MG, ANP |
Interfaces
3 interfaces between chains.
- A–B · 1960 Ų buried · 20 hydrogen bonds · protein-protein
- A–K · 777 Ų buried · 4 hydrogen bonds · protein-protein
- B–K · 755 Ų buried · 7 hydrogen bonds · protein-protein
Compared structures
- 7LVQ · KIF14[391-743] - AMP-PNP closed state class in complex with a microtubule · the same entry in another collection · chain A against A, TM-score 1.00
- L0N7N1 · AlphaFold monomer V2.0 prediction for kinesin-like protein KIF14 (L0N7N1) · a predicted model of the same protein · chain K against A, TM-score 0.94
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q2XVP4
- Every structure of UniProt A0A287AGU7
- Every structure of UniProt L0N7N1
- Elsewhere: RCSB PDB 7LVQ · PDBe 7LVQ · UniProt Q2XVP4 · UniProt A0A287AGU7 · UniProt L0N7N1
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