MolNFT v1 · #178527 · protein
Cryo-EM structure of the tirzepatide-bound human gipr-gs complex
7FIY · Gastric inhibitory polypeptide RECEPTOR,GASTRIC inhibitory…: “Cryo-EM structure of the tirzepatide-bound human gipr-gs complex”. Electron microscopy, 1 ligand (CLR), 19 pockets (best druggability 0.82). MolNFT v1 #178527 on…
- PDB
- 7FIY
- Type
- protein
- Length
- 1,152 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Gastric inhibitory polypeptide RECEPTOR,GASTRIC inhibitory polypeptide RECEPTOR,HUMAN…
- UniProt
- P48546, P04896, P54311, P63212
- Ligands
- CLR
- Pockets
- 19 · best druggability 0.82
Structure
electron microscopy · 6 distinct chains · 40% helix, 24% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| R | Gastric inhibitory polypeptide RECEPTOR,GASTRIC inhibitory polypeptide RECEPTOR,HUMAN glucose-dependent insulinotropic polypeptide receptor | protein | 359 | 71% helix, 5% strand | P48546 |
| P | Tiezepatide | protein | 30 | 90% helix, 0% strand | — |
| A | Guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 235 | 47% helix, 16% strand | P04896 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 341 | 7% helix, 47% strand | P54311 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 58 | 53% helix, 0% strand | P63212 |
| N | Nanobody-35 | protein | 129 | 7% helix, 44% strand | — |
Ligands and binding sites
- CLR · Cholesterol · ligand · 6 copies · chains R
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
19 pockets found on a 1 Å grid, 10 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 830 | 0.82 | 0.85 | 80 | empty |
| 2 | 1052 | 0.77 | 0.79 | 70 | empty |
| 3 | 803 | 0.76 | 0.80 | 50 | empty |
| 4 | 184 | 0.60 | 0.82 | 29 | empty |
| 5 | 258 | 0.60 | 0.80 | 30 | empty |
Interfaces
7 interfaces between chains.
- B–G · 2302 Ų buried · 14 hydrogen bonds · protein-protein
- R–P · 1844 Ų buried · 18 hydrogen bonds · protein-protein
- A–B · 1546 Ų buried · 17 hydrogen bonds · protein-protein
- R–A · 1157 Ų buried · 11 hydrogen bonds · protein-protein
- A–N · 1057 Ų buried · 12 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 disulfide, 2 modified (disulfide, nonstandard).
Compared structures
- 7FIY · Cryo-EM structure of the tirzepatide-bound human GIPR-Gs complex · the same entry in another collection · chain N against N, TM-score 1.00
- P54311 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P04896 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(S) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.84
- P48546 · AlphaFold monomer V2.0 prediction for gastric inhibitory polypeptide receptor (P48546) · a predicted model of the same protein · chain R against A, TM-score 0.82
- P63212 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.67
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P48546
- Every structure of UniProt P04896
- Every structure of UniProt P54311
- Every structure of UniProt P63212
- Elsewhere: RCSB PDB 7FIY · PDBe 7FIY · UniProt P48546 · UniProt P04896 · UniProt P54311 · UniProt P63212
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