MolNFT v1 · #178025 · protein
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-gs complex without NB35
7F24 · Guanine nucleotide-binding protein G(S) subunit alpha…: “Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-gs complex without NB35”. Electron microscopy, 2 ligands (GTP, LDP), 10 pockets (best druggability 0.82).…
- PDB
- 7F24
- Type
- protein
- Length
- 900 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(S) subunit alpha isoforms SHORT,ISOFORM gnas-2 of…
- UniProt
- P63092, P21728, P62873, P59768
- Ligands
- GTP, LDP
- Pockets
- 10 · best druggability 0.82
Structure
electron microscopy · 4 distinct chains · 45% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Guanine nucleotide-binding protein G(S) subunit alpha isoforms SHORT,ISOFORM gnas-2 of guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 226 | 45% helix, 18% strand | P63092 |
| F | D(1A) dopamine receptor | protein | 277 | 86% helix, 0% strand | P21728 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 341 | 9% helix, 46% strand | P62873 |
| D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 56 | 63% helix, 0% strand | P59768 |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
10 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 708 | 0.82 | 0.86 | 56 | empty |
| 2 | 266 | 0.73 | 0.87 | 38 | LDP |
| 3 | 758 | 0.72 | 0.78 | 56 | empty |
| 4 | 253 | 0.58 | 0.79 | 36 | empty |
| 5 | 297 | 0.58 | 0.77 | 38 | empty |
Interfaces
4 interfaces between chains.
- B–D · 2183 Ų buried · 20 hydrogen bonds · protein-protein
- A–F · 1356 Ų buried · 4 hydrogen bonds · protein-protein
- A–B · 985 Ų buried · 6 hydrogen bonds · protein-protein
- F–B · 103 Ų buried · 0 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide (disulfide).
Compared structures
- 7F24 · Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35 · the same entry in another collection · chain A against A, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain D against A, TM-score 0.91
- P21728 · AlphaFold monomer V2.0 prediction for D(1A) dopamine receptor (P21728) · a predicted model of the same protein · chain F against A, TM-score 0.91
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63092
- Every structure of UniProt P21728
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Elsewhere: RCSB PDB 7F24 · PDBe 7F24 · UniProt P63092 · UniProt P21728 · UniProt P62873 · UniProt P59768
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