MolNFT v1 · #177945 · protein
Cryo-EM structure of an activated cholecystokinin a receptor (cckar)- gs complex
7EZK · Chimera of guanine nucleotide-binding protein G(I) subunit…: “Cryo-EM structure of an activated cholecystokinin a receptor (cckar)- gs complex”. Electron microscopy, 9 pockets (best druggability 0.82). MolNFT v1 #177945 on…
- PDB
- 7EZK
- Type
- protein
- Length
- 921 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Chimera of guanine nucleotide-binding protein G(I) subunit alpha-1 and guanine…
- UniProt
- P63096, P63092, P62873, P32238, P59768
- Pockets
- 9 · best druggability 0.82
Structure
electron microscopy · 5 distinct chains · 44% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Chimera of guanine nucleotide-binding protein G(I) subunit alpha-1 and guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 227 | 49% helix, 18% strand | P63096, P63092 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 339 | 7% helix, 48% strand | P62873 |
| D | Cholecystokinin receptor type a | protein | 294 | 83% helix, 4% strand | P32238 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 53 | 49% helix, 0% strand | P59768 |
| P | Cholecystokinin-8 | protein | 8 | 0% helix, 0% strand | — |
Pockets
9 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 558 | 0.82 | 0.88 | 54 | empty |
| 2 | 918 | 0.74 | 0.78 | 67 | empty |
| 3 | 487 | 0.60 | 0.74 | 44 | empty |
| 4 | 117 | 0.42 | 0.76 | 17 | empty |
| 5 | 74 | 0.38 | 0.78 | 20 | empty |
Interfaces
5 interfaces between chains.
- B–G · 2144 Ų buried · 20 hydrogen bonds · protein-protein
- A–B · 1477 Ų buried · 18 hydrogen bonds · protein-protein
- A–D · 1413 Ų buried · 12 hydrogen bonds · protein-protein
- D–P · 979 Ų buried · 15 hydrogen bonds · protein-protein
- B–D · 61 Ų buried · 0 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide, 1 modified (disulfide, sulfation).
Compared structures
- 7EZK · Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gs complex · the same entry in another collection · chain P against P, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P32238 · AlphaFold monomer V2.0 prediction for cholecystokinin receptor type a (P32238) · a predicted model of the same protein · chain D against A, TM-score 0.93
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.90
- P63096 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.74
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63096
- Every structure of UniProt P63092
- Every structure of UniProt P62873
- Every structure of UniProt P32238
- Every structure of UniProt P59768
- Elsewhere: RCSB PDB 7EZK · PDBe 7EZK · UniProt P63096 · UniProt P63092 · UniProt P62873 · UniProt P32238 · UniProt P59768
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