MolNFT v1 · #176955 · protein
Serotonin-bound serotonin 1A (5-HT1A) receptor-gi protein complex
7E2Y · Guanine nucleotide-binding protein G(I) subunit alpha-1: “Serotonin-bound serotonin 1A (5-HT1A) receptor-gi protein complex”. Electron microscopy, 3 ligands (CLR, SRO, J40), 9 pockets (best druggability 0.82). MolNFT v1 #176955 on…
- PDB
- 7E2Y
- Type
- protein
- Length
- 868 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(I) subunit alpha-1; Guanine nucleotide-binding…
- UniProt
- P63096, P62873, P59768, P08908
- Ligands
- CLR, SRO, J40
- Pockets
- 9 · best druggability 0.82
Structure
electron microscopy · 4 distinct chains · 44% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Guanine nucleotide-binding protein G(I) subunit alpha-1 | protein | 219 | 47% helix, 17% strand | P63096 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 328 | 5% helix, 47% strand | P62873 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 45 | 49% helix, 0% strand | P59768 |
| R | Soluble cytochrome B562,5-hydroxytryptamine receptor 1A | protein | 276 | 86% helix, 0% strand | P08908 |
Ligands and binding sites
- CLR · Cholesterol · ligand · 4 copies · chains R
- SRO · Serotonin · ligand · chains R
- J40 · [(2R)-1-[oxidanyl-[(2R,3R,5S,6R)-2,3,5,6-tetrakis(oxidanyl)-4-phosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-3-tetradecanoyloxy-propan-2-yl] (5E, 8E)-hexadeca-5,8,11,14-tetraenoate · ligand · chains A, R
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
9 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 830 | 0.82 | 0.85 | 64 | empty |
| 2 | 555 | 0.78 | 0.84 | 42 | SRO |
| 3 | 277 | 0.66 | 0.82 | 29 | empty |
| 4 | 690 | 0.65 | 0.75 | 57 | empty |
| 5 | 367 | 0.60 | 0.77 | 41 | empty |
Interfaces
4 interfaces between chains.
- B–G · 1670 Ų buried · 16 hydrogen bonds · protein-protein
- A–B · 1216 Ų buried · 6 hydrogen bonds · protein-protein
- A–R · 1100 Ų buried · 9 hydrogen bonds · protein-protein
- B–R · 159 Ų buried · 0 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 disulfide (disulfide).
Compared structures
- 7E2Y · Serotonin-bound Serotonin 1A (5-HT1A) receptor-Gi protein complex · the same entry in another collection · chain R against R, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P08908 · AlphaFold monomer V2.0 prediction for 5-hydroxytryptamine receptor 1A (P08908) · a predicted model of the same protein · chain R against A, TM-score 0.89
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.83
- P63096 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.79
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63096
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Every structure of UniProt P08908
- Elsewhere: RCSB PDB 7E2Y · PDBe 7E2Y · UniProt P63096 · UniProt P62873 · UniProt P59768 · UniProt P08908
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.