A galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 goloco

Structure

x-ray diffraction · 4 distinct chains · 48% helix, 11% strand (DSSP).

Ligands and binding sites

• GDP · Guanosine-5'-diphosphate · ligand · 2 copies · chains A, C, B, D
• SO4 · Sulfate ion · ion · 2 copies · chains A, B
• GOL · Glycerol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

8 pockets found on a 1 Å grid, 5 with a druggability of 0.5 or more.

Interfaces

5 interfaces between chains.

• A–C · 1808 Ų buried · 26 hydrogen bonds · protein-protein
• B–D · 1674 Ų buried · 23 hydrogen bonds · protein-protein
• A–B · 719 Ų buried · 3 hydrogen bonds · protein-protein
• A–D · 273 Ų buried · 1 hydrogen bond · protein-protein
• B–C · 19 Ų buried · 0 hydrogen bonds · protein-protein

Compared structures

• 3QI2 · A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14… · the same entry in another collection · chain D against D, TM-score 1.00
• P63096 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain B against A, TM-score 0.95
• O43566 · AlphaFold monomer V2.0 prediction for regulator of G-protein signaling 14 (O43566) · a predicted model of the same protein · chain C against A, TM-score 0.43

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P63096
• Every structure of UniProt O43566
• Elsewhere: RCSB PDB 3QI2 · PDBe 3QI2 · UniProt P63096 · UniProt O43566