Research workbench
Cryoem structure of somatostatin receptor 4 (SSTR4) in complex with GI1 and its endogeneous ligand sst-14
Analyse Cryoem structure of somatostatin receptor 4 (SSTR4) in complex with GI1 and its… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7XMS
- Type
- protein
- Length
- 1,113 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Somatostatin receptor type 4; Guanine nucleotide-binding protein G(I) subunit alpha-1…
- UniProt
- P31391, P63096, P62873, P59768, P61278
- Pockets
- 17 · best druggability 0.82
Structure
electron microscopy · 6 distinct chains · 29% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| R | Somatostatin receptor type 4 | protein | 276 | 78% helix, 2% strand | P31391 |
| A | Guanine nucleotide-binding protein G(I) subunit alpha-1 | protein | 224 | 39% helix, 16% strand | P63096 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 325 | 0% helix, 44% strand | P62873 |
| C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 45 | 29% helix, 0% strand | P59768 |
| S | Signle chain viable fragment of antibody | protein | 231 | 4% helix, 33% strand | — |
| L | Somatostatin-14 | protein | 12 | 0% helix, 0% strand | P61278 |
Pockets
17 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 642 | 0.82 | 0.87 | 62 | empty |
| 2 | 233 | 0.74 | 0.88 | 48 | empty |
| 3 | 58 | 0.73 | 0.98 | 15 | empty |
| 4 | 777 | 0.73 | 0.78 | 74 | empty |
| 5 | 175 | 0.63 | 0.84 | 26 | empty |
Interfaces
7 interfaces between chains.
- B–C · 1647 Ų buried · 9 hydrogen bonds · protein-protein
- A–B · 1324 Ų buried · 13 hydrogen bonds · protein-protein
- R–A · 1088 Ų buried · 9 hydrogen bonds · protein-protein
- R–L · 905 Ų buried · 6 hydrogen bonds · protein-protein
- A–S · 558 Ų buried · 13 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide (disulfide).
Compared structures
- 7XMS · CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its… · the same entry in another collection · chain L against L, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.98
- P31391 · AlphaFold monomer V2.0 prediction for somatostatin receptor type 4 (P31391) · a predicted model of the same protein · chain R against A, TM-score 0.89
- P63096 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.83
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain C against A, TM-score 0.77
- P61278 · AlphaFold monomer V2.0 prediction for somatostatin (P61278) · a predicted model of the same protein · chain L against A, TM-score 0.22
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P31391
- Every structure of UniProt P63096
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Every structure of UniProt P61278
- Elsewhere: RCSB PDB 7XMS · PDBe 7XMS · UniProt P31391 · UniProt P63096 · UniProt P62873 · UniProt P59768 · UniProt P61278
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