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Cryo-EM structure of mitochondrial calcium uniporter holocomplex in high CA2+

Analyse Cryo-EM structure of mitochondrial calcium uniporter holocomplex in high CA2+ in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 6WDO
Type: protein
Length: 3,645 residues
Chains: 20
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Calcium uniporter protein, mitochondrial; Essential mcu regulator, mitochondrial; Calcium…
UniProt: Q8NE86, Q9H4I9, Q9BPX6, Q8IYU8
Ligands: CA
Pockets: 20 · best druggability 0.83

Structure

electron microscopy · 9 distinct chains · 59% helix, 7% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
G, O	Calcium uniporter protein, mitochondrial	protein	255	57% helix, 9% strand	Q8NE86
H, D, L, P, N	Essential mcu regulator, mitochondrial	protein	51	55% helix, 4% strand	Q9H4I9
C, K	Calcium uniporter protein, mitochondrial	protein	268	62% helix, 10% strand	Q8NE86
A, I	Calcium uniporter protein, mitochondrial	protein	273	59% helix, 10% strand	Q8NE86
B, J	Essential mcu regulator, mitochondrial	protein	53	53% helix, 4% strand	Q9H4I9
E, M	Calcium uniporter protein, mitochondrial	protein	270	59% helix, 11% strand	Q8NE86
F	Essential mcu regulator, mitochondrial	protein	50	54% helix, 4% strand	Q9H4I9
Q, S	Calcium uptake protein 1, mitochondrial	protein	264	61% helix, 2% strand	Q9BPX6
R, T	Calcium uptake protein 2, mitochondrial	protein	287	61% helix, 4% strand	Q8IYU8

Ligands and binding sites

CA · Calcium ion · metal · 2 copies · chains G, C, A, E, I, K, O, M

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	899	0.83	0.85	80	empty
2	939	0.82	0.83	91	empty
3	1025	0.78	0.80	94	empty
4	136	0.77	0.94	32	empty
5	730	0.76	0.81	59	empty

Interfaces

44 interfaces between chains.

I–M · 2396 Å² buried · 12 hydrogen bonds · protein-protein
A–E · 2381 Å² buried · 13 hydrogen bonds · protein-protein
C–A · 2222 Å² buried · 23 hydrogen bonds · protein-protein
I–K · 2194 Å² buried · 25 hydrogen bonds · protein-protein
G–E · 2070 Å² buried · 20 hydrogen bonds · protein-protein

Compared structures

6WDO · Cryo-EM structure of mitochondrial calcium uniporter holocomplex in high Ca2+ · the same entry in another collection · chain J against J, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q8NE86
Every structure of UniProt Q9H4I9
Every structure of UniProt Q9BPX6
Every structure of UniProt Q8IYU8
Elsewhere: RCSB PDB 6WDO · PDBe 6WDO · UniProt Q8NE86 · UniProt Q9H4I9 · UniProt Q9BPX6 · UniProt Q8IYU8

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