Research workbench
Escherichia COLI RNA polymerase rpob H526Y mutant
Analyse Escherichia COLI RNA polymerase rpob H526Y mutant in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5UAQ
- Type
- protein
- Length
- 7,072 residues
- Chains
- 12
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA-directed RNA polymerase subunit alpha; DNA-directed RNA polymerase subunit beta…
- EC
- 2.7.7.6
- UniProt
- P0A7Z4, P0A8V2, P0A8T7, P0A800, P00579
- Ligands
- ZN, MG
- Pockets
- 20 · best druggability 0.84
Structure
x-ray diffraction · 11 distinct chains · 41% helix, 16% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 309 | 29% helix, 22% strand | P0A7Z4 |
| B | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 217 | 19% helix, 29% strand | P0A7Z4 |
| C, I | DNA-directed RNA polymerase subunit beta (EC 2.7.7.6) | protein | 1340 | 32% helix, 23% strand | P0A8V2 |
| D | DNA-directed RNA polymerase subunit beta' (EC 2.7.7.6) | protein | 1166 | 42% helix, 14% strand | P0A8T7 |
| E | DNA-directed RNA polymerase subunit omega (EC 2.7.7.6) | protein | 89 | 66% helix, 0% strand | P0A800 |
| F | RNA polymerase sigma factor rpod | protein | 467 | 67% helix, 3% strand | P00579 |
| G | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 224 | 17% helix, 32% strand | P0A7Z4 |
| H | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 217 | 18% helix, 30% strand | P0A7Z4 |
| J | DNA-directed RNA polymerase subunit beta' (EC 2.7.7.6) | protein | 1155 | 43% helix, 14% strand | P0A8T7 |
| K | DNA-directed RNA polymerase subunit omega (EC 2.7.7.6) | protein | 79 | 62% helix, 0% strand | P0A800 |
| L | RNA polymerase sigma factor rpod | protein | 469 | 67% helix, 3% strand | P00579 |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1054 | 0.84 | 0.85 | 112 | empty |
| 2 | 1091 | 0.82 | 0.83 | 132 | empty |
| 3 | 545 | 0.81 | 0.87 | 64 | empty |
| 4 | 602 | 0.79 | 0.85 | 50 | empty |
| 5 | 167 | 0.78 | 0.94 | 24 | empty |
Interfaces
21 interfaces between chains.
- I–J · 7977 Ų buried · 99 hydrogen bonds · protein-protein
- C–D · 7923 Ų buried · 99 hydrogen bonds · protein-protein
- D–F · 2753 Ų buried · 18 hydrogen bonds · protein-protein
- J–L · 2709 Ų buried · 24 hydrogen bonds · protein-protein
- C–F · 2048 Ų buried · 12 hydrogen bonds · protein-protein
Compared structures
- 5UAQ · Escherichia coli RNA polymerase RpoB H526Y mutant · the same entry in another collection · chain L against L, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A7Z4
- Every structure of UniProt P0A8V2
- Every structure of UniProt P0A8T7
- Every structure of UniProt P0A800
- Every structure of UniProt P00579
- Elsewhere: RCSB PDB 5UAQ · PDBe 5UAQ · UniProt P0A7Z4 · UniProt P0A8V2 · UniProt P0A8T7 · UniProt P0A800 · UniProt P00579
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