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MolNFT v2 · #204771 · protein

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CIII membrane domain)

8BEL: “Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CIII membrane domain)”. Electron microscopy at 2.25, 10 ligands (CDL, HEM, 3PH). MolNFT v2 #204771 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB
8BEL
Type
protein
Length
2,054 residues
Chains
14
Method
electron microscopy
Collection
MolNFT v2 / PDB Bank
Contract
0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
Owner
0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
Resolution
2.25
Source
Arabidopsis thaliana
UniProt
P42792, Q94JS0, Q9FKS5, Q9SG91, Q0WWE3, Q9LXJ2, Q94K78
Ligands
CDL, HEM, 3PH, PC7, PGT, UQ5, PTY, FES, Q7G, UQ7

Structure

electron microscopy · resolution 2.25 · 10 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
C, M—protein387—P42792
D, N—protein179—Q94JS0
E, O—protein244—Q9FKS5
G—protein69—Q9SG91
H—protein64—Q0WWE3
I, S—protein57—Q9LXJ2
J—protein28—Q94K78
Q—protein68—Q9SG91
R—protein63—Q0WWE3
T—protein28—Q94K78

Ligands and binding sites

  • CDL · Cardiolipin · ligand · 7 copies · chains C, D, E, G, M, Q, N, O, S
  • HEM · Protoporphyrin IX containing Fe · ligand · 6 copies · chains C, E, M, O
  • 3PH · 1,2-diacyl-glycerol-3-sn-phosphate · ligand · 6 copies · chains C, G, E, I, M, O, T, N
  • PC7 · (7S)-4-hydroxy-N,N,N-trimethyl-9-oxo-7-[(palmitoyloxy) methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide · ligand · 5 copies · chains C, G, D, I, J, E, M, Q, N, S, T
  • PGT · (1S)-2-{[{[(2R)-2,3-dihydroxypropyl]oxy}(hydroxy) phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate · ligand · 4 copies · chains C, E, G, M
  • UQ5 · 2,3-dimethoxy-5-methyl-6-(3,11,15,19-tetramethyl-eicosa-2,6,10,14,18-pentaenyl)-[1,4]benzoquinone · ligand · 3 copies · chains C, M, N
  • PTY · Phosphatidylethanolamine · ligand · 3 copies · chains I, J, M, Q, S, T
  • FES · Fe2/s2 (inorganic) cluster · ligand · 2 copies · chains D, N
  • Q7G · 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta, 17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside · ligand · 2 copies · chains M
  • UQ7 · Ubiquinone-7 · ligand · chains C, D, M

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • P42792 · AlphaFold monomer V2.0 prediction for cytochrome B (P42792) · a predicted model of the same protein · chain M against A, TM-score 1.00
  • Q9FKS5 · AlphaFold monomer V2.0 prediction for cytochrome C1 2, heme protein, mitochondrial… · a predicted model of the same protein · chain E against A, TM-score 0.99
  • Q0WWE3 · AlphaFold monomer V2.0 prediction for cytochrome B-C1 complex subunit 6-1, mitochondrial… · a predicted model of the same protein · chain R against A, TM-score 0.95
  • Q9SG91 · AlphaFold monomer V2.0 prediction for cytochrome B-C1 complex subunit 8-1, mitochondrial… · a predicted model of the same protein · chain G against A, TM-score 0.92
  • Q9LXJ2 · AlphaFold monomer V2.0 prediction for cytochrome B-C1 complex subunit 9, mitochondrial… · a predicted model of the same protein · chain I against A, TM-score 0.84
  • Q94K78 · AlphaFold monomer V2.0 prediction for cytochrome B-C1 complex subunit 10, mitochondrial… · a predicted model of the same protein · chain J against A, TM-score 0.77
  • Q94JS0 · AlphaFold monomer V2.0 prediction for cytochrome B-C1 complex subunit rieske-1… · a predicted model of the same protein · chain D against A, TM-score 0.70

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