MolNFT v2 · #195156 · protein
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
7T8X · Muscarinic acetylcholine receptor M2,muscarinic…: “Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state”. Electron microscopy at 3.21, 1 ligand (ACH), 10 pockets (best druggability 0.83). MolNFT v2 #195156 on…
- PDB
- 7T8X
- Type
- protein
- Length
- 1,126 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 3.21
- Source
- Homo sapiens; Mus musculus
- Molecule
- Muscarinic acetylcholine receptor M2,muscarinic acetylcholine receptor M2 chimera…
- UniProt
- P08172, P09471, P62873, P59768
- Ligands
- ACH
- Pockets
- 10 · best druggability 0.83
Structure
electron microscopy · resolution 3.21 · 5 distinct chains · 33% helix, 26% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Muscarinic acetylcholine receptor M2,muscarinic acetylcholine receptor M2 chimera | protein | 277 | 78% helix, 0% strand | P08172 |
| B | Guanine nucleotide-binding protein G(o) subunit alpha | protein | 219 | 42% helix, 16% strand | P09471 |
| C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 338 | 8% helix, 46% strand | P62873 |
| D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 56 | 50% helix, 0% strand | P59768 |
| E | Antibody fragment | protein | 236 | 3% helix, 44% strand | — |
Ligands and binding sites
- ACH · Acetylcholine · ligand · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
10 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 878 | 0.83 | 0.85 | 63 | empty |
| 2 | 120 | 0.79 | 0.97 | 20 | ACH |
| 3 | 1162 | 0.71 | 0.75 | 103 | empty |
| 4 | 529 | 0.68 | 0.78 | 76 | empty |
| 5 | 152 | 0.58 | 0.83 | 29 | empty |
Interfaces
6 interfaces between chains.
- C–D · 2137 Ų buried · 12 hydrogen bonds · protein-protein
- B–C · 1107 Ų buried · 7 hydrogen bonds · protein-protein
- A–B · 1047 Ų buried · 3 hydrogen bonds · protein-protein
- B–E · 520 Ų buried · 7 hydrogen bonds · protein-protein
- C–E · 408 Ų buried · 3 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide (disulfide).
Compared structures
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain C against A, TM-score 0.99
- P08172 · AlphaFold monomer V2.0 prediction for muscarinic acetylcholine receptor M2 (P08172) · a predicted model of the same protein · chain A against A, TM-score 0.93
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain D against A, TM-score 0.88
- P09471 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(O) subunit… · a predicted model of the same protein · chain B against A, TM-score 0.77
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P08172
- Every structure of UniProt P09471
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Elsewhere: RCSB PDB 7T8X · PDBe 7T8X · UniProt P08172 · UniProt P09471 · UniProt P62873 · UniProt P59768
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.