Crystal structure of wt H-RAS-gppnhp bound to the rbd of RAF kinase

Structure

x-ray diffraction · 2 distinct chains · 32% helix, 30% strand (DSSP).

Ligands and binding sites

• GNP · Phosphoaminophosphonic acid-guanylate ester · ligand · chains A
• CA · Calcium ion · metal · 2 copies · chains A
• MG · Magnesium ion · metal · chains A
• ACT · Acetate ion · additive · chains A
• DTU · (2R,3S)-1,4-dimercaptobutane-2,3-diol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 668 Ų buried · 14 hydrogen bonds · protein-protein

Compared structures

• 4G0N · Crystal Structure of wt H-Ras-GppNHp bound to the RBD of Raf Kinase · the same entry in another collection · chain A against A, TM-score 1.00
• P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain B against A, TM-score 0.95

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P01112
• Every structure of UniProt P04049
• Elsewhere: RCSB PDB 4G0N · PDBe 4G0N · UniProt P01112 · UniProt P04049