MolNFT v1 · #191441 · protein + DNA
Consensus closed state of RFC:PCNA bound to a 3' SS/DSDNA junction (DNA2)
8DR1 · Replication factor C subunit 1: “Consensus closed state of RFC:PCNA bound to a 3' SS/DSDNA junction (DNA2)”. Electron microscopy, 3 ligands (AGS, GDP, MG), 20 pockets (best druggability 0.84). MolNFT v1 #191441 on GenesisL1: 3D…
- PDB
- 8DR1
- Type
- protein + DNA
- Length
- 2,671 residues
- Chains
- 12
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Replication factor C subunit 1; Replication factor C subunit 4; Replication factor C…
- UniProt
- P38630, P40339, P38629, P40348, P38251, P15873
- Ligands
- AGS, GDP, MG
- Pockets
- 20 · best druggability 0.84
Structure
electron microscopy · 12 distinct chains · 47% helix, 20% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Replication factor C subunit 1 | protein | 493 | 58% helix, 8% strand | P38630 |
| B | Replication factor C subunit 4 | protein | 318 | 61% helix, 9% strand | P40339 |
| C | Replication factor C subunit 3 | protein | 330 | 62% helix, 8% strand | P38629 |
| D | Replication factor C subunit 2 | protein | 343 | 59% helix, 8% strand | P40348 |
| E | Replication factor C subunit 5 | protein | 354 | 56% helix, 13% strand | P38251 |
| F | Proliferating cell nuclear antigen | protein | 258 | 19% helix, 47% strand | P15873 |
| G | Proliferating cell nuclear antigen | protein | 257 | 19% helix, 44% strand | P15873 |
| H | Proliferating cell nuclear antigen | protein | 255 | 19% helix, 44% strand | P15873 |
| I | DNA (5'- D(P*TP*TP*TP*CP*GP*GP*GP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*GP*G)-3') | dna | 19 | — | — |
| J | DNA (5'- D(P*CP*CP*CP*CP*CP*CP*GP*GP*CP*CP*CP*CP*CP*CP*CP*GP*GP*C)-3') | dna | 18 | — | — |
| K | DNA (5'-D(P*TP*TP*AP*GP*GP*GP*GP*GP*GP*GP*GP*GP*A)-3') | dna | 13 | — | — |
| L | DNA (5'-D(P*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*TP*TP*T)-3') | dna | 13 | — | — |
Ligands and binding sites
- AGS · Phosphothiophosphoric acid-adenylate ester · ligand · 4 copies · chains A, B, C, D, E
- GDP · Guanosine-5'-diphosphate · ligand · chains A, E
- MG · Magnesium ion · metal · 4 copies · chains A, B, C, D, E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 597 | 0.84 | 0.89 | 71 | empty |
| 2 | 976 | 0.83 | 0.84 | 88 | empty |
| 3 | 362 | 0.82 | 0.91 | 57 | GDP |
| 4 | 174 | 0.81 | 0.96 | 32 | MG, AGS |
| 5 | 176 | 0.80 | 0.96 | 33 | MG, AGS |
Interfaces
36 interfaces between chains.
- A–E · 3125 Ų buried · 36 hydrogen bonds · protein-protein
- D–E · 2965 Ų buried · 41 hydrogen bonds · protein-protein
- B–C · 2599 Ų buried · 37 hydrogen bonds · protein-protein
- C–D · 2575 Ų buried · 33 hydrogen bonds · protein-protein
- A–B · 2378 Ų buried · 31 hydrogen bonds · protein-protein
Compared structures
- 8DR1 · Consensus closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) · the same entry in another collection · chain I against I, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P38630
- Every structure of UniProt P40339
- Every structure of UniProt P38629
- Every structure of UniProt P40348
- Every structure of UniProt P38251
- Every structure of UniProt P15873
- Elsewhere: RCSB PDB 8DR1 · PDBe 8DR1 · UniProt P38630 · UniProt P40339 · UniProt P38629 · UniProt P40348 · UniProt P38251 · UniProt P15873
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