MolNFT v1 · #190583 · protein

Cryo-EM structure of the galanin-bound GALR2-minigq complex

7XJK: “Cryo-EM structure of the galanin-bound GALR2-minigq complex”. Electron microscopy, 12 pockets (best druggability 0.81). MolNFT v1 #190583 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7XJK
Type: protein
Length: 1,139 residues
Chains: 6
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Galanin; Guanine nucleotide-binding protein G(Q); Guanine nucleotide-binding protein…
UniProt: P22466, P62873, P59768, O43603
Pockets: 12 · best druggability 0.81

Structure

electron microscopy · 6 distinct chains · 35% helix, 27% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Galanin	protein	16	50% helix, 0% strand	P22466
B	Guanine nucleotide-binding protein G(Q)	protein	226	46% helix, 17% strand	—
C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	protein	338	7% helix, 45% strand	P62873
D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	protein	51	57% helix, 0% strand	P59768
E	Single Fab chain (SVFV16)	protein	230	3% helix, 47% strand	—
F	Galanin receptor type 2	protein	278	79% helix, 4% strand	O43603

Pockets

12 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	755	0.81	0.84	55	empty
2	108	0.76	0.96	28	empty
3	1146	0.75	0.78	99	empty
4	281	0.55	0.76	48	empty
5	64	0.54	0.87	17	empty

Interfaces

7 interfaces between chains.

C–D · 1981 Å² buried · 14 hydrogen bonds · protein-protein
B–C · 1193 Å² buried · 7 hydrogen bonds · protein-protein
B–F · 1117 Å² buried · 10 hydrogen bonds · protein-protein
A–F · 913 Å² buried · 10 hydrogen bonds · protein-protein
B–E · 577 Å² buried · 8 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 disulfide (disulfide).

Compared structures

7XJK · Cryo-EM structure of the galanin-bound GALR2-miniGq complex · the same entry in another collection · chain F against F, TM-score 1.00
P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain C against A, TM-score 0.99
O43603 · AlphaFold monomer V2.0 prediction for galanin receptor type 2 (O43603) · a predicted model of the same protein · chain F against A, TM-score 0.93
P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain D against A, TM-score 0.90
P22466 · AlphaFold monomer V2.0 prediction for galanin peptides (P22466) · a predicted model of the same protein · chain A against A, TM-score 0.44

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P22466
Every structure of UniProt P62873
Every structure of UniProt P59768
Every structure of UniProt O43603
Elsewhere: RCSB PDB 7XJK · PDBe 7XJK · UniProt P22466 · UniProt P62873 · UniProt P59768 · UniProt O43603

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