MolNFT v1 · #177980 · protein
Cryo-EM structure of dopamine receptor 1 and mini-gs complex with dopamine bound
7F0T · D(1A) dopamine receptor: “Cryo-EM structure of dopamine receptor 1 and mini-gs complex with dopamine bound”. Electron microscopy, 1 ligand (LDP), 13 pockets (best druggability 0.80). MolNFT v1 #177980 on GenesisL1: 3D structure…
- PDB
- 7F0T
- Type
- protein
- Length
- 1,033 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- D(1A) dopamine receptor; Guanine nucleotide-binding protein G(S) subunit alpha isoforms…
- UniProt
- P21728, P63092, P62873, P59768
- Ligands
- LDP
- Pockets
- 13 · best druggability 0.80
Structure
electron microscopy · 5 distinct chains · 40% helix, 24% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| F | D(1A) dopamine receptor | protein | 282 | 84% helix, 1% strand | P21728 |
| A | Guanine nucleotide-binding protein G(S) subunit alpha isoforms SHORT,ISOFORM gnas-2 of guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 228 | 49% helix, 17% strand | P63092 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 339 | 9% helix, 47% strand | P62873 |
| D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 56 | 57% helix, 0% strand | P59768 |
| E | NANOBODY35 | protein | 128 | 2% helix, 41% strand | — |
Ligands and binding sites
- LDP · L-dopamine · ligand · chains F
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
13 pockets found on a 1 Å grid, 9 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 631 | 0.80 | 0.85 | 54 | empty |
| 2 | 1091 | 0.76 | 0.79 | 72 | empty |
| 3 | 923 | 0.75 | 0.78 | 63 | empty |
| 4 | 509 | 0.74 | 0.82 | 55 | empty |
| 5 | 283 | 0.66 | 0.82 | 36 | LDP |
Interfaces
6 interfaces between chains.
- B–D · 2288 Ų buried · 18 hydrogen bonds · protein-protein
- F–A · 1721 Ų buried · 10 hydrogen bonds · protein-protein
- A–B · 1483 Ų buried · 23 hydrogen bonds · protein-protein
- A–E · 788 Ų buried · 9 hydrogen bonds · protein-protein
- B–E · 608 Ų buried · 7 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide (disulfide).
Compared structures
- 7F0T · Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound · the same entry in another collection · chain E against E, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 1.00
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain D against A, TM-score 0.94
- P21728 · AlphaFold monomer V2.0 prediction for D(1A) dopamine receptor (P21728) · a predicted model of the same protein · chain F against A, TM-score 0.89
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P21728
- Every structure of UniProt P63092
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Elsewhere: RCSB PDB 7F0T · PDBe 7F0T · UniProt P21728 · UniProt P63092 · UniProt P62873 · UniProt P59768
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.