MolNFT v1 · #177942 · protein
Cryo-EM structure of an activated cholecystokinin a receptor (cckar)- gi complex
7EZH · Guanine nucleotide-binding protein G(I) subunit alpha-1: “Cryo-EM structure of an activated cholecystokinin a receptor (cckar)- gi complex”. Electron microscopy, 12 pockets (best druggability 0.82). MolNFT v1 #177942 on GenesisL1…
- PDB
- 7EZH
- Type
- protein
- Length
- 1,149 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(I) subunit alpha-1; Guanine nucleotide-binding…
- UniProt
- P63096, P62873, P32238, P59768
- Pockets
- 12 · best druggability 0.82
Structure
electron microscopy · 6 distinct chains · 36% helix, 28% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Guanine nucleotide-binding protein G(I) subunit alpha-1 | protein | 217 | 50% helix, 18% strand | P63096 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 339 | 8% helix, 46% strand | P62873 |
| D | Cholecystokinin receptor type a | protein | 296 | 82% helix, 5% strand | P32238 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 56 | 46% helix, 0% strand | P59768 |
| H | SCFV16 | protein | 233 | 5% helix, 47% strand | — |
| P | Cholecystokinin-8 | protein | 8 | 0% helix, 0% strand | — |
Pockets
12 pockets found on a 1 Å grid, 5 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 678 | 0.82 | 0.86 | 61 | empty |
| 2 | 1038 | 0.72 | 0.76 | 75 | empty |
| 3 | 232 | 0.60 | 0.80 | 25 | empty |
| 4 | 197 | 0.54 | 0.79 | 21 | empty |
| 5 | 118 | 0.53 | 0.82 | 23 | empty |
Interfaces
7 interfaces between chains.
- B–G · 2095 Ų buried · 21 hydrogen bonds · protein-protein
- A–B · 1256 Ų buried · 13 hydrogen bonds · protein-protein
- A–D · 1165 Ų buried · 10 hydrogen bonds · protein-protein
- D–P · 986 Ų buried · 11 hydrogen bonds · protein-protein
- A–H · 542 Ų buried · 6 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 disulfide, 1 modified (disulfide, sulfation).
Compared structures
- 7EZH · Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gi complex · the same entry in another collection · chain P against P, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P32238 · AlphaFold monomer V2.0 prediction for cholecystokinin receptor type a (P32238) · a predicted model of the same protein · chain D against A, TM-score 0.94
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.93
- P63096 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.82
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63096
- Every structure of UniProt P62873
- Every structure of UniProt P32238
- Every structure of UniProt P59768
- Elsewhere: RCSB PDB 7EZH · PDBe 7EZH · UniProt P63096 · UniProt P62873 · UniProt P32238 · UniProt P59768
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