MolNFT v1 · #177454 · protein
Structure of the ALPHA2A-adrenergic receptor goa signaling complex
7EJ0 · Guanine nucleotide-binding protein G(O) subunit alpha: “Structure of the ALPHA2A-adrenergic receptor goa signaling complex”. Electron microscopy, 1 ligand (E5E), 19 pockets (best druggability 0.82). MolNFT v1 #177454 on GenesisL1…
- PDB
- 7EJ0
- Type
- protein
- Length
- 1,099 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(O) subunit alpha; Guanine nucleotide-binding protein…
- UniProt
- P09471, P62873, P59768, P08913
- Ligands
- E5E
- Pockets
- 19 · best druggability 0.82
Structure
electron microscopy · 5 distinct chains · 31% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Guanine nucleotide-binding protein G(O) subunit alpha | protein | 217 | 40% helix, 16% strand | P09471 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 335 | 5% helix, 36% strand | P62873 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 54 | 52% helix, 0% strand | P59768 |
| H | SCFV16 | protein | 233 | 3% helix, 41% strand | — |
| R | Alpha-2A adrenergic receptor | protein | 260 | 77% helix, 0% strand | P08913 |
Ligands and binding sites
- E5E · Noradrenaline · ligand · chains R
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
19 pockets found on a 1 Å grid, 10 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 852 | 0.82 | 0.84 | 68 | empty |
| 2 | 624 | 0.75 | 0.82 | 72 | empty |
| 3 | 479 | 0.75 | 0.83 | 44 | E5E |
| 4 | 127 | 0.74 | 0.93 | 25 | empty |
| 5 | 66 | 0.72 | 0.97 | 19 | empty |
Interfaces
6 interfaces between chains.
- B–G · 1824 Ų buried · 8 hydrogen bonds · protein-protein
- A–B · 1171 Ų buried · 12 hydrogen bonds · protein-protein
- A–R · 973 Ų buried · 2 hydrogen bonds · protein-protein
- A–H · 587 Ų buried · 9 hydrogen bonds · protein-protein
- B–H · 416 Ų buried · 1 hydrogen bond · protein-protein
Modifications
Observed in the structure: 4 disulfide (disulfide).
Compared structures
- 7EJ0 · Structure of the alpha2A-adrenergic receptor GoA signaling complex · the same entry in another collection · chain R against R, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.98
- P08913 · AlphaFold monomer V2.0 prediction for alpha-2A adrenergic receptor (P08913) · a predicted model of the same protein · chain R against A, TM-score 0.91
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.86
- P09471 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(O) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.76
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P09471
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Every structure of UniProt P08913
- Elsewhere: RCSB PDB 7EJ0 · PDBe 7EJ0 · UniProt P09471 · UniProt P62873 · UniProt P59768 · UniProt P08913
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