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MolNFT v1 · #169736 · protein + DNA + RNA

Cryo-EM structure of E. COLI rnap-promoter initial transcribing complex with 5-nt RNA transcript (rpitc-5NT)

6XLL: “Cryo-EM structure of E. COLI rnap-promoter initial transcribing complex with 5-nt RNA transcript (rpitc-5NT)”. Electron microscopy, 3 ligands (1N7, ZN, MG). MolNFT v1 #169736 on GenesisL1: 3D structure, binding sites and pockets in…

PDB
6XLL
Type
protein + DNA + RNA
Length
3,786 residues
Chains
9
Method
electron microscopy
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
UniProt
P0A7Z6, B7MIX3, P0A8T8, B7MFL0, P00579
Ligands
1N7, ZN, MG

Structure

electron microscopy · 9 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—protein231—P0A7Z6
B—protein222—P0A7Z6
C—protein1339—B7MIX3
D—protein1336—P0A8T8
E—protein79—B7MFL0
F—protein476—P00579
N—dna50——
T—dna48——
R—rna5——

Ligands and binding sites

  • 1N7 · Chapso · ligand · 2 copies · chains C, A
  • ZN · Zinc ion · metal · 2 copies · chains D
  • MG · Magnesium ion · metal · chains D, R

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6XLL · Cryo-EM structure of E. coli RNAP-promoter initial transcribing complex with 5-nt RNA… · the same entry in another collection · chain R against R, TM-score 1.00
  • B7MIX3 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta (B7MIX3) · a predicted model of the same protein · chain C against A, TM-score 0.99
  • P0A7Z6 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit alpha (P0A7Z6) · a predicted model of the same protein · chain B against A, TM-score 0.96
  • B7MFL0 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit omega (B7MFL0) · a predicted model of the same protein · chain E against A, TM-score 0.95

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