Human 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide and cotylenin a

Structure

x-ray diffraction · 3 distinct chains · 80% helix, 0% strand (DSSP).

Ligands and binding sites

• 1F5 · (1R,3aS,4R,5R,6R,9aR,10E)-6-({(1S,2R,4S,5R,6R,8S,9S)-5-hydroxy-2-(methoxymethyl)-9-methyl-9-[(2S)-oxiran-2-yl]-3,7,10,1 1-tetraoxatricyclo[6.2.1.0~1,6~]undec-4-yl}oxy)-1-(methoxymethyl)-4,9a-dimethyl-7-(propan-2-yl)-1,2,3,3a,4,5,6,8,9,9a-de cahydrodicyclopenta[a,d][8]annulene-1,5-diol · ligand · 2 copies · chains A, P, B
• K · Potassium ion · metal · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

3 interfaces between chains.

• A–B · 985 Ų buried · 13 hydrogen bonds · protein-protein
• B–P · 585 Ų buried · 13 hydrogen bonds · protein-protein
• A–P · 577 Ų buried · 16 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 modified (phosphorylation).

Compared structures

• 4IHL · Human 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide and Cotylenin A · the same entry in another collection · chain A against A, TM-score 1.00
• P63104 · AlphaFold monomer V2.0 prediction for 14-3-3 protein ZETA/DELTA (P63104) · a predicted model of the same protein · chain A against A, TM-score 0.99
• P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain P against A, TM-score 0.25

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P63104
• Every structure of UniProt P04049
• Elsewhere: RCSB PDB 4IHL · PDBe 4IHL · UniProt P63104 · UniProt P04049