Research workbench
Cryoem structure of E.COLI RNA polymerase elongation complex bound to nusa and nusg (nusa and nusg elongation complex in more-swiveled conformation)
Analyse Cryoem structure of E.COLI RNA polymerase elongation complex bound to nusa and… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7PY7
- Type
- protein + DNA + RNA
- Length
- 3,901 residues
- Chains
- 10
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Ntdna; Tdna; RNA; DNA-directed RNA polymerase subunit alpha; DNA-directed RNA polymerase…
- EC
- 2.7.7.6
- UniProt
- P0A7Z4, P0A8V4, P0A8T8, P0A800, C3SID2, D7Y526
- Ligands
- ZN, MG
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 10 distinct chains · 31% helix, 19% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| N | Ntdna | dna | 27 | — | — |
| T | Tdna | dna | 31 | — | — |
| R | RNA | rna | 11 | — | — |
| A | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 228 | 22% helix, 32% strand | P0A7Z4 |
| B | DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) | protein | 220 | 20% helix, 36% strand | P0A7Z4 |
| C | DNA-directed RNA polymerase subunit beta (EC 2.7.7.6) | protein | 1341 | 30% helix, 22% strand | P0A8V4 |
| D | DNA-directed RNA polymerase subunit beta' (EC 2.7.7.6) | protein | 1335 | 37% helix, 17% strand | P0A8T8 |
| E | DNA-directed RNA polymerase subunit omega (EC 2.7.7.6) | protein | 90 | 67% helix, 0% strand | P0A800 |
| G | Transcription TERMINATION/ANTITERMINATION protein nusg | protein | 123 | 30% helix, 20% strand | C3SID2 |
| F | Transcription TERMINATION/ANTITERMINATION protein nusa | protein | 495 | 23% helix, 5% strand | D7Y526 |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1042 | 0.85 | 0.86 | 111 | empty |
| 2 | 1029 | 0.81 | 0.83 | 101 | empty |
| 3 | 438 | 0.81 | 0.90 | 53 | empty |
| 4 | 561 | 0.81 | 0.87 | 68 | empty |
| 5 | 446 | 0.80 | 0.88 | 67 | empty |
Interfaces
21 interfaces between chains.
- C–D · 8377 Ų buried · 82 hydrogen bonds · protein-protein
- A–B · 2029 Ų buried · 18 hydrogen bonds · protein-protein
- A–C · 1899 Ų buried · 25 hydrogen bonds · protein-protein
- D–E · 1268 Ų buried · 12 hydrogen bonds · protein-protein
- B–D · 1150 Ų buried · 3 hydrogen bonds · protein-protein
Compared structures
- 7PY7 · CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA… · the same entry in another collection · chain F against F, TM-score 1.00
- P0A8T8 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta' (P0A8T8) · a predicted model of the same protein · chain D against A, TM-score 0.93
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A7Z4
- Every structure of UniProt P0A8V4
- Every structure of UniProt P0A8T8
- Every structure of UniProt P0A800
- Every structure of UniProt C3SID2
- Every structure of UniProt D7Y526
- Elsewhere: RCSB PDB 7PY7 · PDBe 7PY7 · UniProt P0A7Z4 · UniProt P0A8V4 · UniProt P0A8T8 · UniProt P0A800 · UniProt C3SID2 · UniProt D7Y526
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.