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Four-site variant (Y222C, C197S, C432S, C433S) of E. COLI hydrogenase- 2

Analyse Four-site variant (Y222C, C197S, C432S, C433S) of E. COLI hydrogenase- 2 in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB
6G7M
Type
protein
Length
1,638 residues
Chains
4
Method
x-ray diffraction
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
UniProt
P69741, P0ACE0
Ligands
SF4, F3S, FCO, MG, NI

Structure

x-ray diffraction · 2 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
S, T—protein268—P69741
L, M—protein551—P0ACE0

Ligands and binding sites

  • SF4 · Iron/sulfur cluster · ligand · 4 copies · chains S, L, T, M
  • F3S · FE3-S4 cluster · ligand · 2 copies · chains S, L, T, M
  • FCO · Carbonmonoxide-(dicyano) iron · ligand · 2 copies · chains L, M
  • MG · Magnesium ion · metal · 4 copies · chains L, M
  • NI · Nickel (II) ion · metal · 2 copies · chains L, M

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6G7M · Four-site variant (Y222C, C197S, C432S, C433S) of E. coli hydrogenase-2 · the same entry in another collection · chain S against S, TM-score 1.00
  • P0ACE0 · AlphaFold monomer V2.0 prediction for hydrogenase-2 large chain (P0ACE0) · a predicted model of the same protein · chain L against A, TM-score 1.00
  • P69741 · AlphaFold monomer V2.0 prediction for hydrogenase-2 small chain (P69741) · a predicted model of the same protein · chain S against A, TM-score 1.00

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