Research workbench
Yeast 20S proteasome with human BETA5I (1-138) and human BETA6 (97- 111; 118-133) in complex with thiazole based inhibitor RO19
Analyse Yeast 20S proteasome with human BETA5I (1-138) and human BETA6 (97- 111… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5M2B
- Type
- protein
- Length
- 6,342 residues
- Chains
- 28
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Proteasome subunit alpha type-2; Proteasome subunit alpha type-3; Proteasome subunit…
- EC
- 3.4.25.1
- UniProt
- P23639, P23638, P40303, P32379, P40302, P21242, P21243, P25043, P25451, P22141, P28062, P30656, P23724, Q59GN1, P30657, P38624
- Ligands
- 7DX, MG, CL
- Pockets
- 20 · best druggability 0.82
Structure
x-ray diffraction · 14 distinct chains · 35% helix, 29% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, O | Proteasome subunit alpha type-2 (EC 3.4.25.1) | protein | 250 | 36% helix, 25% strand | P23639 |
| B, P | Proteasome subunit alpha type-3 (EC 3.4.25.1) | protein | 244 | 38% helix, 25% strand | P23638 |
| C, Q | Proteasome subunit alpha type-4 (EC 3.4.25.1) | protein | 240 | 36% helix, 23% strand | P40303 |
| D, R | Proteasome subunit alpha type-5 (EC 3.4.25.1) | protein | 235 | 38% helix, 27% strand | P32379 |
| E, S | Proteasome subunit alpha type-6 (EC 3.4.25.1) | protein | 231 | 38% helix, 28% strand | P40302 |
| F, T | Probable proteasome subunit alpha type-7 (EC 3.4.25.1) | protein | 243 | 40% helix, 26% strand | P21242 |
| G, U | Proteasome subunit alpha type-1 (EC 3.4.25.1) | protein | 241 | 40% helix, 23% strand | P21243 |
| H, V | Proteasome subunit beta type-2 (EC 3.4.25.1) | protein | 226 | 29% helix, 34% strand | P25043 |
| I, W | Proteasome subunit beta type-3 (EC 3.4.25.1) | protein | 204 | 32% helix, 32% strand | P25451 |
| J, X | Proteasome subunit beta type-4 (EC 3.4.25.1) | protein | 195 | 34% helix, 34% strand | P22141 |
| K, Y | Proteasome subunit beta type-8,PROTEASOME subunit beta type-5 (EC 3.4.25.1) | protein | 211 | 36% helix, 34% strand | P28062, P30656 |
| L, Z | Proteasome subunit beta type-6,PROTEASOME subunit beta TYPE,PROTEASOME subunit beta type-6,PROTEASOME subunit beta type, proteasome subunit beta type-6 (EC 3.4.25.1) | protein | 222 | 28% helix, 31% strand | P23724, Q59GN1 |
| M, a | Proteasome subunit beta type-7 (EC 3.4.25.1) | protein | 233 | 31% helix, 31% strand | P30657 |
| N, b | Proteasome subunit beta type-1 (EC 3.4.25.1) | protein | 196 | 36% helix, 34% strand | P38624 |
Ligands and binding sites
- 7DX · (2~{S})-2-cyclohexyl-4-oxidanylidene-4-[[7-(4-phenyl-1, 3-thiazol-2-yl)quinolin-2-yl]amino]butanoic acid · ligand · 2 copies · chains K, L, Y, Z
- MG · Magnesium ion · metal · 7 copies · chains G, I, Y, J, K, W, L, V, N, a, H, Z
- CL · Chloride ion · ion · 4 copies · chains G, H, N, U, V, b
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 921 | 0.82 | 0.84 | 127 | empty |
| 2 | 1052 | 0.80 | 0.81 | 111 | empty |
| 3 | 906 | 0.80 | 0.82 | 111 | empty |
| 4 | 918 | 0.80 | 0.82 | 104 | empty |
| 5 | 1066 | 0.79 | 0.80 | 126 | empty |
Interfaces
92 interfaces between chains.
- H–I · 1708 Ų buried · 29 hydrogen bonds · protein-protein
- V–W · 1692 Ų buried · 29 hydrogen bonds · protein-protein
- B–C · 1606 Ų buried · 29 hydrogen bonds · protein-protein
- P–Q · 1598 Ų buried · 24 hydrogen bonds · protein-protein
- L–V · 1533 Ų buried · 20 hydrogen bonds · protein-protein
Compared structures
- 5M2B · Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in… · the same entry in another collection · chain C against C, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P23639
- Every structure of UniProt P23638
- Every structure of UniProt P40303
- Every structure of UniProt P32379
- Every structure of UniProt P40302
- Every structure of UniProt P21242
- Every structure of UniProt P21243
- Every structure of UniProt P25043
- Every structure of UniProt P25451
- Every structure of UniProt P22141
- Every structure of UniProt P28062
- Every structure of UniProt P30656
- Every structure of UniProt P23724
- Every structure of UniProt Q59GN1
- Every structure of UniProt P30657
- Every structure of UniProt P38624
- Elsewhere: RCSB PDB 5M2B · PDBe 5M2B · UniProt P23639 · UniProt P23638 · UniProt P40303 · UniProt P32379 · UniProt P40302 · UniProt P21242 · UniProt P21243 · UniProt P25043 · UniProt P25451 · UniProt P22141 · UniProt P28062 · UniProt P30656 · UniProt P23724 · UniProt Q59GN1 · UniProt P30657 · UniProt P38624
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