Research workbench
Cryo-EM structure of MTORC1
Analyse Cryo-EM structure of MTORC1 in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5H64
- Type
- protein
- Length
- 6,430 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- SERINE/THREONINE-protein kinase mtor; Regulatory-associated protein of mtor; Target of…
- EC
- 2.7.11.1
- UniProt
- P42345, Q8N122, Q9BVC4
- Pockets
- 20 · best druggability 0.86
Structure
electron microscopy · 3 distinct chains · 52% helix, 8% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, a | SERINE/THREONINE-protein kinase mtor (EC 2.7.11.1) | protein | 2101 | 65% helix, 2% strand | P42345 |
| B, b | Regulatory-associated protein of mtor | protein | 797 | 38% helix, 14% strand | Q8N122 |
| C, c | Target of rapamycin complex subunit LST8 | protein | 317 | 2% helix, 37% strand | Q9BVC4 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 504 | 0.86 | 0.92 | 77 | empty |
| 2 | 562 | 0.86 | 0.91 | 83 | empty |
| 3 | 718 | 0.85 | 0.88 | 119 | empty |
| 4 | 1093 | 0.84 | 0.84 | 78 | empty |
| 5 | 1131 | 0.83 | 0.84 | 79 | empty |
Interfaces
7 interfaces between chains.
- A–a · 1794 Ų buried · 22 hydrogen bonds · protein-protein
- A–C · 824 Ų buried · 17 hydrogen bonds · protein-protein
- a–c · 819 Ų buried · 17 hydrogen bonds · protein-protein
- A–b · 607 Ų buried · 13 hydrogen bonds · protein-protein
- B–a · 595 Ų buried · 12 hydrogen bonds · protein-protein
Compared structures
- 5H64 · Cryo-EM structure of mTORC1 · the same entry in another collection · chain c against c, TM-score 1.00
- Q9BVC4 · AlphaFold monomer V2.0 prediction for target of rapamycin complex subunit LST8 (Q9BVC4) · a predicted model of the same protein · chain C against A, TM-score 0.94
- P42345 · AlphaFold monomer V2.0 prediction for SERINE/THREONINE-protein kinase mtor (P42345) · a predicted model of the same protein · chain A against A, TM-score 0.86
- Q8N122 · AlphaFold monomer V2.0 prediction for regulatory-associated protein of mtor (Q8N122) · a predicted model of the same protein · chain B against A, TM-score 0.77
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P42345
- Every structure of UniProt Q8N122
- Every structure of UniProt Q9BVC4
- Elsewhere: RCSB PDB 5H64 · PDBe 5H64 · UniProt P42345 · UniProt Q8N122 · UniProt Q9BVC4
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