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Crystal structure of a transcribing RNA polymerase II complex reveals a complete transcription bubble

Analyse Crystal structure of a transcribing RNA polymerase II complex reveals a… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 5C44
Type: protein + DNA + RNA
Length: 4,029 residues
Chains: 15
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II subunit RPB2…
EC: 2.7.7.6
UniProt: P04050, P08518, P16370, P20433, P20434, P20435, P34087, P20436, P27999, P22139, P38902, P40422
Pockets: 20 · best druggability 0.86

Structure

x-ray diffraction · 15 distinct chains · 30% helix, 20% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6)	protein	1434	38% helix, 15% strand	P04050
B	DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6)	protein	1158	27% helix, 21% strand	P08518
C	DNA-directed RNA polymerase II subunit RPB3	protein	265	21% helix, 19% strand	P16370
D	DNA-directed RNA polymerase II subunit RPB4	protein	178	48% helix, 4% strand	P20433
E	DNA-directed RNA polymerases I, II, and III subunit RPABC1	protein	214	38% helix, 20% strand	P20434
F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	protein	87	30% helix, 16% strand	P20435
G	DNA-directed RNA polymerase II subunit RPB7	protein	171	8% helix, 46% strand	P34087
H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	protein	135	0% helix, 49% strand	P20436
I	DNA-directed RNA polymerase II subunit RPB9	protein	114	4% helix, 26% strand	P27999
J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	protein	65	48% helix, 2% strand	P22139
K	DNA-directed RNA polymerase II subunit RPB11	protein	115	39% helix, 23% strand	P38902
L	DNA-directed RNA polymerases I, II, and III subunit RPABC4	protein	44	0% helix, 11% strand	P40422
R	Synthetic RNA	rna	9	—	—
S	Synthetic DNA	dna	13	—	—
U	Synthetic DNA	dna	27	—	—

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1141	0.86	0.88	127	empty
2	982	0.84	0.85	104	empty
3	851	0.83	0.86	97	empty
4	425	0.82	0.90	62	empty
5	1008	0.82	0.83	92	empty

Interfaces

39 interfaces between chains.

A–B · 9857 Å² buried · 149 hydrogen bonds · protein-protein
D–G · 2547 Å² buried · 32 hydrogen bonds · protein-protein
A–E · 2415 Å² buried · 25 hydrogen bonds · protein-protein
A–F · 2323 Å² buried · 20 hydrogen bonds · protein-protein
C–K · 2163 Å² buried · 15 hydrogen bonds · protein-protein

Compared structures

5C44 · Crystal structure of a transcribing RNA Polymerase II complex reveals a complete… · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P04050
Every structure of UniProt P08518
Every structure of UniProt P16370
Every structure of UniProt P20433
Every structure of UniProt P20434
Every structure of UniProt P20435
Every structure of UniProt P34087
Every structure of UniProt P20436
Every structure of UniProt P27999
Every structure of UniProt P22139
Every structure of UniProt P38902
Every structure of UniProt P40422
Elsewhere: RCSB PDB 5C44 · PDBe 5C44 · UniProt P04050 · UniProt P08518 · UniProt P16370 · UniProt P20433 · UniProt P20434 · UniProt P20435 · UniProt P34087 · UniProt P20436 · UniProt P27999 · UniProt P22139 · UniProt P38902 · UniProt P40422

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