Crystal structure of norrin in complex with the cysteine-rich domain of frizzled 4 and sucrose octasulfate

Structure

x-ray diffraction · 2 distinct chains · 21% helix, 31% strand (DSSP).

Ligands and binding sites

• GU4 · 2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose · ligand · chains B, A
• YYJ · 1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose · ligand · chains B, A
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 2 copies · chains B, A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

1 pocket found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• B–A · 853 Ų buried · 7 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 9 disulfide, 2 glycan (disulfide, N-glycosylation).

Compared structures

• 5BQC · Crystal structure of Norrin in complex with the cysteine-rich domain of Frizzled 4 and… · the same entry in another collection · chain B against B, TM-score 1.00
• Q9ULV1 · AlphaFold monomer V2.0 prediction for frizzled-4 (Q9ULV1) · a predicted model of the same protein · chain B against A, TM-score 0.94
• Q00604 · AlphaFold monomer V2.0 prediction for norrin (Q00604) · a predicted model of the same protein · chain A against A, TM-score 0.93

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q9ULV1
• Every structure of UniProt Q00604
• Elsewhere: RCSB PDB 5BQC · PDBe 5BQC · UniProt Q9ULV1 · UniProt Q00604