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Kinetics by X-ray crystallography: native E2.MGF42-.2K+ crystal for rb+ bound crystals
Analyse Kinetics by X-ray crystallography: native E2.MGF42-.2K+ crystal for rb+ bound… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5AW9
- Type
- protein
- Length
- 1,299 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Na, K-ATPase alpha subunit; NA+,K+-ATPase beta subunit; Phospholemman-like protein
- UniProt
- Q4H132, C4IX13, Q70Q12
- Ligands
- MF4, CLR, NAG, K, MG
- Pockets
- 20 · best druggability 0.79
Structure
x-ray diffraction · 3 distinct chains · 44% helix, 16% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Na, K-ATPase alpha subunit | protein | 992 | 49% helix, 15% strand | Q4H132 |
| B | NA+,K+-ATPase beta subunit | protein | 268 | 20% helix, 19% strand | C4IX13 |
| G | Phospholemman-like protein | protein | 39 | 69% helix, 0% strand | Q70Q12 |
Ligands and binding sites
- MF4 · Tetrafluoromagnesate(2-) · ligand · chains A
- CLR · Cholesterol · ligand · chains A, B
- NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 3 copies · chains B
- K · Potassium ion · metal · 3 copies · chains A
- MG · Magnesium ion · metal · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 888 | 0.79 | 0.81 | 59 | empty |
| 2 | 575 | 0.72 | 0.80 | 73 | K |
| 3 | 62 | 0.71 | 0.96 | 13 | empty |
| 4 | 124 | 0.66 | 0.89 | 25 | empty |
| 5 | 557 | 0.64 | 0.76 | 68 | empty |
Interfaces
3 interfaces between chains.
- A–B · 2220 Ų buried · 35 hydrogen bonds · protein-protein
- A–G · 813 Ų buried · 1 hydrogen bond · protein-protein
- B–G · 532 Ų buried · 12 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide, 2 glycan (disulfide, N-glycosylation).
Compared structures
- 5AW9 · Kinetics by X-ray crystallography: native E2.MgF42-.2K+ crystal for Rb+ bound crystals · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q4H132
- Every structure of UniProt C4IX13
- Every structure of UniProt Q70Q12
- Elsewhere: RCSB PDB 5AW9 · PDBe 5AW9 · UniProt Q4H132 · UniProt C4IX13 · UniProt Q70Q12
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