MolNFT v1 · #190328 · protein
Tethered peptide activation mechanism of adhesion gpcrs ADGRG2 and ADGRG4
7WUI · Mini-gs: “Tethered peptide activation mechanism of adhesion gpcrs ADGRG2 and ADGRG4”. Electron microscopy, 12 pockets (best druggability 0.80). MolNFT v1 #190328 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 7WUI
- Type
- protein
- Length
- 1,225 residues
- Chains
- 7
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Mini-gs; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Guanine…
- UniProt
- P62873, P59768, Q8CJ12
- Pockets
- 12 · best druggability 0.80
Structure
electron microscopy · 7 distinct chains · 29% helix, 27% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Mini-gs | protein | 230 | 45% helix, 14% strand | — |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 338 | 7% helix, 46% strand | P62873 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 55 | 44% helix, 0% strand | P59768 |
| N | Nanobody-35 | protein | 126 | 2% helix, 36% strand | — |
| R | Adhesion G-protein coupled receptor G2,MCHERRY | protein | 245 | 78% helix, 2% strand | Q8CJ12 |
| S | SCFV16 | protein | 220 | 4% helix, 40% strand | — |
| L | IP15 | protein | 11 | 27% helix, 0% strand | — |
Pockets
12 pockets found on a 1 Å grid, 5 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1043 | 0.80 | 0.81 | 69 | empty |
| 2 | 399 | 0.78 | 0.87 | 48 | empty |
| 3 | 543 | 0.76 | 0.83 | 57 | empty |
| 4 | 320 | 0.62 | 0.79 | 40 | empty |
| 5 | 131 | 0.53 | 0.81 | 28 | empty |
Interfaces
9 interfaces between chains.
- B–G · 2263 Ų buried · 21 hydrogen bonds · protein-protein
- A–B · 1649 Ų buried · 25 hydrogen bonds · protein-protein
- A–R · 1485 Ų buried · 10 hydrogen bonds · protein-protein
- A–N · 985 Ų buried · 20 hydrogen bonds · protein-protein
- R–L · 967 Ų buried · 13 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide, 1 modified (disulfide, nonstandard).
Compared structures
- 7WUI · Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4 · the same entry in another collection · chain L against L, TM-score 1.00
- P62873 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.98
- Q8CJ12 · AlphaFold monomer V2.0 prediction for adhesion G-protein coupled receptor G2 (Q8CJ12) · a predicted model of the same protein · chain R against A, TM-score 0.91
- P59768 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.83
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P62873
- Every structure of UniProt P59768
- Every structure of UniProt Q8CJ12
- Elsewhere: RCSB PDB 7WUI · PDBe 7WUI · UniProt P62873 · UniProt P59768 · UniProt Q8CJ12
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