MolNFT v1 · #188428 · protein + DNA + RNA

Human telomerase catalytic core rnp with H2A/H2B

7TRF · Histone H2B type 1-C/E/F/G/I: “Human telomerase catalytic core rnp with H2A/H2B”. Electron microscopy, 20 pockets (best druggability 0.86). MolNFT v1 #188428 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7TRF
Type: protein + DNA + RNA
Length: 1,330 residues
Chains: 5
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Histone H2B type 1-C/E/F/G/I; Histone H2A; Telomerase RNA, partial sequence; Telomerase…
EC: 2.7.7.49
UniProt: P62807, A0A024RAS2, O14746
Pockets: 20 · best druggability 0.86

Structure

electron microscopy · 5 distinct chains · 51% helix, 14% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
F	Histone H2B type 1-C/E/F/G/I	protein	90	77% helix, 3% strand	P62807
E	Histone H2A	protein	82	67% helix, 4% strand	A0A024RAS2
B	Telomerase RNA, partial sequence	rna	217	—	—
A	Telomerase reverse transcriptase (EC 2.7.7.49)	protein	934	47% helix, 16% strand	O14746
D	Telomeric repeat substrate	dna	7	—	—

Pockets

20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1158	0.86	0.87	94	empty
2	1085	0.79	0.81	72	empty
3	855	0.77	0.81	48	empty
4	1079	0.74	0.77	63	empty
5	118	0.72	0.92	30	empty

Interfaces

6 interfaces between chains.

B–A · 6163 Å² buried · 104 hydrogen bonds · protein-nucleic
F–E · 2475 Å² buried · 13 hydrogen bonds · protein-protein
A–D · 755 Å² buried · 10 hydrogen bonds · protein-nucleic
F–B · 482 Å² buried · 6 hydrogen bonds · protein-nucleic
E–B · 411 Å² buried · 4 hydrogen bonds · protein-nucleic

Compared structures

7TRF · Human telomerase catalytic core RNP with H2A/H2B · the same entry in another collection · chain E against E, TM-score 1.00
O14746 · AlphaFold monomer V2.0 prediction for telomerase reverse transcriptase (O14746) · a predicted model of the same protein · chain A against A, TM-score 0.94
P62807 · AlphaFold monomer V2.0 prediction for histone H2B type 1-C/E/F/G/I (P62807) · a predicted model of the same protein · chain F against A, TM-score 0.82

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P62807
Every structure of UniProt A0A024RAS2
Every structure of UniProt O14746
Elsewhere: RCSB PDB 7TRF · PDBe 7TRF · UniProt P62807 · UniProt A0A024RAS2 · UniProt O14746

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