MolNFT v1 · #177872 · protein
Cryo-EM structure of human GPR158 in complex with RGS7-GBETA5 in a 2:1:1 ratio
7EWP · Probable G-protein coupled receptor 158: “Cryo-EM structure of human GPR158 in complex with RGS7-GBETA5 in a 2:1:1 ratio”. Electron microscopy, 20 pockets (best druggability 0.87). MolNFT v1 #177872 on GenesisL1: 3D structure…
- PDB
- 7EWP
- Type
- protein
- Length
- 1,816 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Probable G-protein coupled receptor 158; Regulator of G-protein signaling 7; Guanine…
- UniProt
- Q5T848, P49802, O14775
- Pockets
- 20 · best druggability 0.87
Structure
electron microscopy · 4 distinct chains · 43% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Probable G-protein coupled receptor 158 | protein | 577 | 50% helix, 8% strand | Q5T848 |
| B | Probable G-protein coupled receptor 158 | protein | 504 | 47% helix, 12% strand | Q5T848 |
| C | Regulator of G-protein signaling 7 | protein | 396 | 57% helix, 3% strand | P49802 |
| D | Guanine nucleotide-binding protein subunit beta-5 | protein | 339 | 7% helix, 36% strand | O14775 |
Pockets
20 pockets found on a 1 Å grid, 18 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1019 | 0.87 | 0.88 | 101 | empty |
| 2 | 1044 | 0.85 | 0.86 | 133 | empty |
| 3 | 969 | 0.81 | 0.82 | 117 | empty |
| 4 | 1008 | 0.81 | 0.81 | 60 | empty |
| 5 | 324 | 0.76 | 0.87 | 62 | empty |
Interfaces
3 interfaces between chains.
- C–D · 3591 Ų buried · 20 hydrogen bonds · protein-protein
- A–B · 1826 Ų buried · 10 hydrogen bonds · protein-protein
- A–C · 1319 Ų buried · 2 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 12 disulfide (disulfide).
Compared structures
- 7EWP · Cryo-EM structure of human GPR158 in complex with RGS7-Gbeta5 in a 2:1:1 ratio · the same entry in another collection · chain D against D, TM-score 1.00
- P49802 · AlphaFold monomer V2.0 prediction for regulator of G-protein signaling 7 (P49802) · a predicted model of the same protein · chain C against A, TM-score 0.96
- O14775 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein subunit beta-5… · a predicted model of the same protein · chain D against A, TM-score 0.95
- Q5T848 · AlphaFold monomer V2.0 prediction for probable G-protein coupled receptor 158 (Q5T848) · a predicted model of the same protein · chain B against A, TM-score 0.83
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q5T848
- Every structure of UniProt P49802
- Every structure of UniProt O14775
- Elsewhere: RCSB PDB 7EWP · PDBe 7EWP · UniProt Q5T848 · UniProt P49802 · UniProt O14775
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