Cryo-EM structure of CA2+-free HSSLO1-BETA4 channel complex

Structure

electron microscopy · 2 distinct chains · 43% helix, 13% strand (DSSP).

Ligands and binding sites

• PGW · (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy) phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate · ligand · 40 copies · chains A, E, B, D, H, F, C, G
• CLR · Cholesterol · ligand · 8 copies · chains A, E, B, F, C, G, D, H
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 8 copies · chains E, F, G, H

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Interfaces

18 interfaces between chains.

• C–D · 1957 Ų buried · 12 hydrogen bonds · protein-protein
• A–B · 1936 Ų buried · 12 hydrogen bonds · protein-protein
• B–C · 1923 Ų buried · 12 hydrogen bonds · protein-protein
• A–D · 1917 Ų buried · 12 hydrogen bonds · protein-protein
• A–E · 1057 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 16 disulfide, 8 covalent, 8 glycan (disulfide, covalent ligand, N-glycosylation).

Compared structures

• 6V35 · Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex · the same entry in another collection · chain H against H, TM-score 1.00
• Q12791 · AlphaFold monomer V2.0 prediction for calcium-activated potassium channel subunit alpha-1… · a predicted model of the same protein · chain A against A, TM-score 0.94
• Q86W47 · AlphaFold monomer V2.0 prediction for calcium-activated potassium channel subunit beta-4… · a predicted model of the same protein · chain E against A, TM-score 0.86

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q12791
• Every structure of UniProt Q86W47
• Elsewhere: RCSB PDB 6V35 · PDBe 6V35 · UniProt Q12791 · UniProt Q86W47