Crystal structure of protein phosphatase 1 (PP1) bound to the muscle glycogen-targeting subunit (gm)

Structure

x-ray diffraction · 3 distinct chains · 32% helix, 21% strand (DSSP).

Ligands and binding sites

• 1ZN · (2S,3S,4E,6E,8S,9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid · ligand · chains A, C
• FGA · Gamma-D-glutamic acid · ligand · chains A, C
• DAM · N-methyl-alpha-beta-dehydroalanine · ligand · chains A, C

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

2 interfaces between chains.

• A–B · 1132 Ų buried · 16 hydrogen bonds · protein-protein
• A–C · 292 Ų buried · 4 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 3 covalent, 1 d-amino, 1 modified (covalent ligand, D-amino acid, nonstandard).

Compared structures

• 6DNO · Crystal structure of Protein Phosphatase 1 (PP1) bound to the muscle glycogen-targeting… · the same entry in another collection · chain A against A, TM-score 1.00
• P62136 · AlphaFold monomer V2.0 prediction for SERINE/THREONINE-protein phosphatase PP1-alpha… · a predicted model of the same protein · chain A against A, TM-score 0.99
• Q00756 · AlphaFold monomer V2.0 prediction for protein phosphatase 1 regulatory subunit 3A (Q00756) · a predicted model of the same protein · chain B against A, TM-score 0.22

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P62136
• Every structure of UniProt Q00756
• Elsewhere: RCSB PDB 6DNO · PDBe 6DNO · UniProt P62136 · UniProt Q00756