MolNFT v1 · #143690 · protein
Cryo-EM structure of human insulin degrading enzyme in complex with Fab H11-E heavy chain, Fab H11-E light chain and insulin
6B70 · Insulin-degrading enzyme: “Cryo-EM structure of human insulin degrading enzyme in complex with Fab H11-E heavy chain, Fab H11-E light…”. Electron microscopy, 20 pockets (best druggability 0.85). MolNFT v1 #143690 on GenesisL1: 3D…
- PDB
- 6B70
- Type
- protein
- Length
- 2,783 residues
- Chains
- 8
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Insulin-degrading enzyme; Fab H11-E heavy chain; Fab H11-E light chain; Insulin
- EC
- 3.4.24.56
- UniProt
- P14735, P0DOX5, Q6GMX0, P01308
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 6 distinct chains · 33% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Insulin-degrading enzyme (EC 3.4.24.56) | protein | 949 | 40% helix, 17% strand | P14735 |
| B | Insulin-degrading enzyme (EC 3.4.24.56) | protein | 940 | 41% helix, 17% strand | P14735 |
| C, E | Fab H11-E heavy chain | protein | 218 | 4% helix, 49% strand | P0DOX5 |
| D, F | Fab H11-E light chain | protein | 211 | 5% helix, 43% strand | Q6GMX0 |
| a | Insulin | protein | 21 | 0% helix, 5% strand | P01308 |
| c | Insulin | protein | 15 | 0% helix, 7% strand | P01308 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 597 | 0.85 | 0.90 | 51 | empty |
| 2 | 1028 | 0.83 | 0.84 | 84 | empty |
| 3 | 1013 | 0.83 | 0.84 | 100 | empty |
| 4 | 1143 | 0.81 | 0.82 | 119 | empty |
| 5 | 488 | 0.72 | 0.81 | 65 | empty |
Interfaces
9 interfaces between chains.
- E–F · 1851 Ų buried · 8 hydrogen bonds · protein-protein
- C–D · 1731 Ų buried · 3 hydrogen bonds · protein-protein
- A–B · 1655 Ų buried · 16 hydrogen bonds · protein-protein
- A–a · 1267 Ų buried · 17 hydrogen bonds · protein-protein
- B–c · 1021 Ų buried · 11 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 10 disulfide (disulfide).
Compared structures
- 6B70 · Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy… · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P14735
- Every structure of UniProt P0DOX5
- Every structure of UniProt Q6GMX0
- Every structure of UniProt P01308
- Elsewhere: RCSB PDB 6B70 · PDBe 6B70 · UniProt P14735 · UniProt P0DOX5 · UniProt Q6GMX0 · UniProt P01308
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