MolNFT v1 · #134691 · protein
Crystal structure of tryptophan synthase from M. tuberculosis - ligand-free form, trpa-G66V mutant
5TCH · Tryptophan synthase alpha chain: “Crystal structure of tryptophan synthase from M. tuberculosis - ligand-free form, trpa-G66V mutant”. X-ray diffraction, 20 pockets (best druggability 0.88). MolNFT v1 #134691 on GenesisL1: 3D…
- PDB
- 5TCH
- Type
- protein
- Length
- 2,598 residues
- Chains
- 8
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Tryptophan synthase alpha chain; Tryptophan synthase beta chain
- EC
- 4.2.1.20
- UniProt
- P9WFY1, P9WFX9
- Pockets
- 20 · best druggability 0.88
Structure
x-ray diffraction · 8 distinct chains · 49% helix, 18% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Tryptophan synthase alpha chain (EC 4.2.1.20) | protein | 249 | 51% helix, 16% strand | P9WFY1 |
| B | Tryptophan synthase beta chain (EC 4.2.1.20) | protein | 405 | 47% helix, 21% strand | P9WFX9 |
| G | Tryptophan synthase alpha chain (EC 4.2.1.20) | protein | 247 | 53% helix, 15% strand | P9WFY1 |
| H | Tryptophan synthase beta chain (EC 4.2.1.20) | protein | 406 | 46% helix, 19% strand | P9WFX9 |
| E | Tryptophan synthase alpha chain (EC 4.2.1.20) | protein | 246 | 52% helix, 15% strand | P9WFY1 |
| F | Tryptophan synthase beta chain (EC 4.2.1.20) | protein | 399 | 48% helix, 21% strand | P9WFX9 |
| C | Tryptophan synthase alpha chain (EC 4.2.1.20) | protein | 248 | 52% helix, 15% strand | P9WFY1 |
| D | Tryptophan synthase beta chain (EC 4.2.1.20) | protein | 398 | 49% helix, 20% strand | P9WFX9 |
Ligands and binding sites
- FMT · Formic acid · additive · 20 copies · chains A, B, G, H, F, E, D, C
- MLI · Malonate ion · additive · 8 copies · chains A, B, G, H, E, F, C, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 13 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 991 | 0.88 | 0.90 | 112 | empty |
| 2 | 1038 | 0.86 | 0.88 | 131 | MLI |
| 3 | 1105 | 0.86 | 0.87 | 149 | MLI |
| 4 | 59 | 0.74 | 0.99 | 15 | empty |
| 5 | 436 | 0.74 | 0.83 | 57 | MLI, FMT |
Interfaces
10 interfaces between chains.
- B–D · 1831 Ų buried · 25 hydrogen bonds · protein-protein
- H–F · 1824 Ų buried · 27 hydrogen bonds · protein-protein
- A–B · 1452 Ų buried · 25 hydrogen bonds · protein-protein
- G–H · 1439 Ų buried · 27 hydrogen bonds · protein-protein
- E–F · 1370 Ų buried · 28 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 4 modified (cofactor).
Compared structures
- 5TCH · Crystal structure of tryptophan synthase from M. tuberculosis - ligand-free form… · the same entry in another collection · chain D against D, TM-score 1.00
- P9WFY1 · AlphaFold monomer V2.0 prediction for tryptophan synthase alpha chain (P9WFY1) · a predicted model of the same protein · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P9WFY1
- Every structure of UniProt P9WFX9
- Elsewhere: RCSB PDB 5TCH · PDBe 5TCH · UniProt P9WFY1 · UniProt P9WFX9
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