Research workbench
14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide
Analyse 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 4FJ3
- Type
- protein
- Length
- 456 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 14-3-3 protein ZETA/DELTA; RAF proto-oncogene SERINE/THREONINE-protein kinase
- EC
- 2.7.11.1
- UniProt
- P63104, P04049
- Pockets
- 3 · best druggability 0.60
Structure
x-ray diffraction · 3 distinct chains · 82% helix, 0% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 14-3-3 protein ZETA/DELTA | protein | 220 | 84% helix, 0% strand | P63104 |
| B | 14-3-3 protein ZETA/DELTA | protein | 222 | 86% helix, 0% strand | P63104 |
| P | RAF proto-oncogene SERINE/THREONINE-protein kinase (EC 2.7.11.1) | protein | 14 | 0% helix, 0% strand | P04049 |
Pockets
3 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 131 | 0.60 | 0.85 | 23 | empty |
| 2 | 218 | 0.53 | 0.77 | 33 | empty |
| 3 | 246 | 0.49 | 0.75 | 26 | empty |
Interfaces
3 interfaces between chains.
- A–B · 949 Ų buried · 10 hydrogen bonds · protein-protein
- A–P · 591 Ų buried · 13 hydrogen bonds · protein-protein
- B–P · 452 Ų buried · 13 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 modified (phosphorylation).
Compared structures
- 4FJ3 · 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide · the same entry in another collection · chain A against A, TM-score 1.00
- P63104 · AlphaFold monomer V2.0 prediction for 14-3-3 protein ZETA/DELTA (P63104) · a predicted model of the same protein · chain B against A, TM-score 0.99
- P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain P against A, TM-score 0.35
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63104
- Every structure of UniProt P04049
- Elsewhere: RCSB PDB 4FJ3 · PDBe 4FJ3 · UniProt P63104 · UniProt P04049
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