Research workbench
Pharmacological chaperones for human alpha-N-acetylgalactosaminidase
Analyse Pharmacological chaperones for human alpha-N-acetylgalactosaminidase in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 4DO4
- Type
- protein
- Length
- 787 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Alpha-N-acetylgalactosaminidase
- EC
- 3.2.1.49
- UniProt
- P17050
- Ligands
- DJN, NAG, MAN, BMA, FUC
- Pockets
- 6 · best druggability 0.56
Structure
x-ray diffraction · 2 distinct chains · 33% helix, 24% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Alpha-N-acetylgalactosaminidase (EC 3.2.1.49) | protein | 387 | 33% helix, 25% strand | P17050 |
| B | Alpha-N-acetylgalactosaminidase (EC 3.2.1.49) | protein | 400 | 33% helix, 23% strand | P17050 |
Ligands and binding sites
- DJN · N-[(3S,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl) piperidin-3-yl]acetamide · ligand · 2 copies · chains A, B
- NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 10 copies · chains A, B
- MAN · alpha-D-mannopyranose · sugar · 4 copies
- BMA · beta-D-mannopyranose · sugar · 3 copies
- FUC · alpha-L-fucopyranose · sugar
- GOL · Glycerol · additive · 13 copies · chains A, B
- CIT · Citric acid · additive · 2 copies · chains A, B
- ACY · Acetic acid · additive · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
6 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 281 | 0.56 | 0.77 | 50 | GOL |
| 2 | 267 | 0.56 | 0.77 | 34 | empty |
| 3 | 58 | 0.42 | 0.82 | 18 | DJN, GOL, ACY |
| 4 | 67 | 0.39 | 0.80 | 20 | DJN, GOL |
| 5 | 87 | 0.38 | 0.77 | 16 | empty |
Interfaces
1 interface between chains.
- A–B · 1013 Ų buried · 8 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 8 disulfide, 6 glycan (disulfide, N-glycosylation).
Compared structures
- 4DO4 · Pharmacological chaperones for human alpha-N-acetylgalactosaminidase · the same entry in another collection · chain A against A, TM-score 1.00
- P17050 · AlphaFold monomer V2.0 prediction for alpha-N-acetylgalactosaminidase (P17050) · a predicted model of the same protein · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P17050
- Elsewhere: RCSB PDB 4DO4 · PDBe 4DO4 · UniProt P17050
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