Research workbench
Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.
Analyse Studies of a constitutively active G-alpha subunit provide insights into the… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3V00
- Type
- protein
- Length
- 1,046 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(T) subunit alpha-1/ guanine nucleotide-binding…
- UniProt
- P04695, P10824
- Ligands
- GDP
- Pockets
- 16 · best druggability 0.77
Structure
x-ray diffraction · 3 distinct chains · 46% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C | Guanine nucleotide-binding protein G(T) subunit alpha-1/ guanine nucleotide-binding protein G(I) subunit alpha-1 chimeric protein | protein | 353 | 45% helix, 12% strand | P04695, P10824 |
| B | Guanine nucleotide-binding protein G(T) subunit alpha-1/ guanine nucleotide-binding protein G(I) subunit alpha-1 chimeric protein | protein | 342 | 44% helix, 13% strand | P04695, P10824 |
| A | Guanine nucleotide-binding protein G(T) subunit alpha-1/ guanine nucleotide-binding protein G(I) subunit alpha-1 chimeric protein | protein | 351 | 48% helix, 13% strand | P04695, P10824 |
Ligands and binding sites
- GDP · Guanosine-5'-diphosphate · ligand · 3 copies · chains C, B, A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
16 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 877 | 0.77 | 0.80 | 83 | empty |
| 2 | 596 | 0.76 | 0.83 | 64 | GDP |
| 3 | 563 | 0.74 | 0.82 | 69 | GDP |
| 4 | 256 | 0.72 | 0.87 | 38 | GDP |
| 5 | 455 | 0.67 | 0.79 | 63 | empty |
Interfaces
3 interfaces between chains.
- C–B · 1214 Ų buried · 17 hydrogen bonds · protein-protein
- B–A · 1103 Ų buried · 9 hydrogen bonds · protein-protein
- C–A · 146 Ų buried · 0 hydrogen bonds · protein-protein
Compared structures
- 3V00 · Studies of a constitutively active G-alpha subunit provide insights into the mechanism of… · the same entry in another collection · chain C against C, TM-score 1.00
- P10824 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I) subunit… · a predicted model of the same protein · chain B against A, TM-score 0.93
- P04695 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(T) subunit… · a predicted model of the same protein · chain B against A, TM-score 0.91
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P04695
- Every structure of UniProt P10824
- Elsewhere: RCSB PDB 3V00 · PDBe 3V00 · UniProt P04695 · UniProt P10824
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