Research workbench
Heterotrimeric complex of a gt-ALPHA/GI-alpha chimera and the gt-beta-gamma subunits
Analyse Heterotrimeric complex of a gt-ALPHA/GI-alpha chimera and the gt-beta-gamma… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1GOT
- Type
- protein
- Length
- 735 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Gt-ALPHA/GI-alpha chimera; Gt-beta; Gt-gamma
- UniProt
- P04695, P62871, P02698
- Ligands
- GDP
- Pockets
- 4 · best druggability 0.81
Structure
x-ray diffraction · 3 distinct chains · 33% helix, 29% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Gt-ALPHA/GI-alpha chimera | protein | 338 | 53% helix, 14% strand | P04695 |
| B | Gt-beta | protein | 339 | 8% helix, 48% strand | P62871 |
| G | Gt-gamma | protein | 58 | 69% helix, 0% strand | P02698 |
Ligands and binding sites
- GDP · Guanosine-5'-diphosphate · ligand · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 795 | 0.81 | 0.85 | 66 | empty |
| 2 | 896 | 0.76 | 0.79 | 80 | GDP |
| 3 | 398 | 0.63 | 0.78 | 59 | empty |
| 4 | 269 | 0.55 | 0.76 | 34 | empty |
Interfaces
2 interfaces between chains.
- B–G · 2442 Ų buried · 27 hydrogen bonds · protein-protein
- A–B · 1302 Ų buried · 22 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 12 modified (engineered).
Compared structures
- 1GOT · Heterotrimeric complex of a gt-ALPHA/GI-alpha chimera and the gt-beta-gamma subunits · the same entry in another collection · chain A against A, TM-score 1.00
- P62871 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P04695 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(T) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.95
- P02698 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(T) subunit… · a predicted model of the same protein · chain G against A, TM-score 0.92
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P04695
- Every structure of UniProt P62871
- Every structure of UniProt P02698
- Elsewhere: RCSB PDB 1GOT · PDBe 1GOT · UniProt P04695 · UniProt P62871 · UniProt P02698
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