Research workbench
PHOB(E)-SIGMA70(4)-(rnap-betha-flap-tip-helix)-DNA transcription activation sub-complex
Analyse PHOB(E)-SIGMA70(4)-(rnap-betha-flap-tip-helix)-DNA transcription activation… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3T72
- Type
- protein + DNA
- Length
- 3,258 residues
- Chains
- 50
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Phosphate regulon transcriptional regulatory protein phob; Pho box DNA (strand 1); Pho…
- EC
- 2.7.7.6
- UniProt
- P0AFJ5, P00579, E8Y6A0
- Pockets
- 20 · best druggability 0.56
Structure
x-ray diffraction · 4 distinct chains · 0% helix, 0% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B, E, F, I, J, M, N, R, S, V, W, Z, 1, 4, 5, 8, 9, c, d, g, h, k, l | Phosphate regulon transcriptional regulatory protein phob | protein | 102 | 0% helix, 0% strand | P0AFJ5 |
| C, G, K, O, T, X, 2, 6, a, e, i, m | Pho box DNA (strand 1) | dna | 26 | — | — |
| D, H, L, P, U, Y, 3, 7, b, f, j, n | Pho box DNA (strand 2) | dna | 26 | — | — |
| o, q | RNA polymerase sigma factor rpod, DNA-directed RNA polymerase subunit beta (EC 2.7.7.6) | protein | 93 | 0% helix, 0% strand | P00579, E8Y6A0 |
Pockets
20 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 379 | 0.56 | 0.74 | 37 | empty |
| 2 | 263 | 0.51 | 0.74 | 30 | empty |
| 3 | 208 | 0.45 | 0.73 | 27 | empty |
| 4 | 186 | 0.44 | 0.73 | 28 | empty |
| 5 | 168 | 0.41 | 0.73 | 26 | empty |
Interfaces
82 interfaces between chains.
- i–j · 1056 Ų buried · 91 hydrogen bonds · nucleic-nucleic
- a–b · 1055 Ų buried · 93 hydrogen bonds · nucleic-nucleic
- e–f · 1054 Ų buried · 93 hydrogen bonds · nucleic-nucleic
- m–n · 1053 Ų buried · 93 hydrogen bonds · nucleic-nucleic
- 6–7 · 1051 Ų buried · 100 hydrogen bonds · nucleic-nucleic
Compared structures
- 3T72 · PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex · the same entry in another collection · chain F against F, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0AFJ5
- Every structure of UniProt P00579
- Every structure of UniProt E8Y6A0
- Elsewhere: RCSB PDB 3T72 · PDBe 3T72 · UniProt P0AFJ5 · UniProt P00579 · UniProt E8Y6A0
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